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Literature summary extracted from

  • Stourman, N.V.; Branch, M.C.; Schaab, M.R.; Harp, J.M.; Ladner, J.E.; Armstrong, R.N.
    Structure and function of YghU, a nu-class glutathione transferase related to YfcG from Escherichia coli (2011), Biochemistry, 50, 1274-1281.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 gene yghU, expression in Escherichia coli strain BL21(DE3), expression of the selenomethionine YghU derivative in Escherichia coli strain B834 DE3 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.18 purified recombinant YghU, hanging-drop vapor diffusion method, YghU at 20 mg/ml in 30 mM NaH2PO4, pH 7.0, 1 mM DTT, and 20 mM GSH, is mixed with an equal volume of reservoir solution containing 0.1 M Bis-Tris, 0.2 M NaCl, pH 5.5, 25% w/v PEG 3350, 25°C, 1 week, X-ray diffraction structure determination and analysis at 1.5-2.5 A resolution Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 oxidized glutathione
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Escherichia coli
-
 gene yghU
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant YghU from strain BL21(DE3) by anion and cation exchange chromatography and dialysis Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Escherichia coli S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 additional information YghU from Escherichia coli binds two molecules of GSH in each active site. YghU exhibits little or no GSH transferase activity with most typical electrophilic substrates but does possess a modest catalytic activity toward several organic hydroperoxides. The enzyme also exhibits disulfide-bond reductase activity towards 2-hydroxyethyl disulfide Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSH transferase homologue
-
 Escherichia coli
2.5.1.18 nu-class glutathione transferase
-
 Escherichia coli
2.5.1.18 YghU
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5 7 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
2.5.1.18 evolution structures and properties of YghU and YfcG indicate that they are members of the same distinct subfamily of GSH transferase homologues, proposed to be called the Nu-class GSH transferases. Cytoscape cluster analysis of the GSH transferase superfamily, overvew Escherichia coli