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Literature summary extracted from

  • Navdaeva, V.; Zurbriggen, A.; Waltersperger, S.; Schneider, P.; Oberholzer, A.; Baehler, P.; Baechler, C.; Grieder, A.; Baumann, U.; Erni, B.
    Phosphoenolpyruvate: sugar phosphotransferase system from the hyperthermophilic Thermoanaerobacter tengcongensis (2011), Biochemistry, 50, 1184-1193.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.9 expressed in Escherichia coli K-12 and Rosetta (DE3) cells Caldanaerobacter subterraneus subsp. tengcongensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.9 C-terminal domain of enzyme I in complex with phosphoenolpyruvate, sitting drop vapor diffusion method, using 0.1 M succinic acid (pH 7.0), and 15% PEG3350 at 20°C Caldanaerobacter subterraneus subsp. tengcongensis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.9 C502A active site mutant Caldanaerobacter subterraneus subsp. tengcongensis
2.7.3.9 H189A active site mutant Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.9 38165
-
 x * 38165, electrospray ionization mass spectrometry Caldanaerobacter subterraneus subsp. tengcongensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.9 Caldanaerobacter subterraneus subsp. tengcongensis Q8R7R4
-
-
2.7.3.9 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 Q8R7R4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.3.9 phosphoprotein phosphorylation of the N-terminal domain of enzyme I at His189 is catalyzed by the C-terminal domain of enzyme I Caldanaerobacter subterraneus subsp. tengcongensis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.9 Ni2+-NTA column chromatography and gel filtration Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.9 phosphoenolpyruvate + protein histidine
-
 Caldanaerobacter subterraneus subsp. tengcongensis pyruvate + protein Npi-phospho-L-histidine
-
 ?
2.7.3.9 phosphoenolpyruvate + protein histidine
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 pyruvate + protein Npi-phospho-L-histidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.9 ? x * 38165, electrospray ionization mass spectrometry Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

EC Number Synonyms Comment Organism
2.7.3.9 EIC C-terminal domain of enzyme I Caldanaerobacter subterraneus subsp. tengcongensis
2.7.3.9 More enzyme I is part of the phosphoenolpyruvate: sugar phosphotransferase system Caldanaerobacter subterraneus subsp. tengcongensis
2.7.3.9 ptsA
-
 Caldanaerobacter subterraneus subsp. tengcongensis