Literature summary extracted from
Tian, H.; Guan, R.; Salsi, E.; Campanini, B.; Bettati, S.; Kumar, V.P.; Karsten, W.E.; Mozzarelli, A.; Cook, P.F.
Identification of the structural determinants for the stability of substrate and aminoacrylate external Schiff bases in O-acetylserine sulfhydrylase-A (2010), Biochemistry, 49, 6093-6103.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.5.1.47 |
N71A |
mutant exhibits formation of the alpha-aminoacrylate intermediate, but the rate constant for its formation from the external Schiff base is decreased by 1 order of magnitude compared to that of the wild type |
Salmonella enterica |
2.5.1.47 |
Q142A |
mutant is unable to form the alpha-aminoacrylate intermediate but produces pyruvate at a rate much greater than that of the wild-type enzyme |
Salmonella enterica |
2.5.1.47 |
S69A |
mutant exhibits formation of the alpha-aminoacrylate intermediate, but the rate constant for its formation from the external Schiff base is decreased by 1 order of magnitude compared to that of the wild type |
Salmonella enterica |
2.5.1.47 |
T68A |
mutant is unable to form the alpha-aminoacrylate intermediate but produces pyruvate at a rate much greater than that of the wild-type enzyme |
Salmonella enterica |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.47 |
Salmonella enterica |
P0A1E3 |
subsp. enterica serovar Typhimurium |
- |