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Literature summary extracted from

  • Glover, K.; Weerapana, E.; Chen, M.; Imperiali, B.
    Direct biochemical evidence for the utilization of UDP-bacillosamine by PglC, an essential glycosyl-1-phosphate transferase in the Campylobacter jejuni N-linked glycosylation pathway (2006), Biochemistry, 45, 5343-5350.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.36 His-tagged protein expressed in Escherichia coli BL21-CodonPlus (DE3)RIL Campylobacter jejuni

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.36 additional information not inhibited by tunicamycin Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.36 0.0043
-
 UDP-6-hydroxybacillosamine pH 8.0, 20°C (room temperature) Campylobacter jejuni
2.7.8.36 0.0062
-
 UDP-bacillosamine pH 8.0, 20°C (room temperature) Campylobacter jejuni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.36 membrane
-
 Campylobacter jejuni 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.36 Mg2+
-
 Campylobacter jejuni

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.36 Campylobacter jejuni
-
-
-
2.7.8.36 Campylobacter jejuni ATCC 700819D
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.36 preparation of membrane fraction, immobilized metal ion affinity chromatography (Ni2+) Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.36 UDP-6-hydroxybacillosamine + undecaprenyl phosphate
-
 Campylobacter jejuni 6-hydroxybacillosaminyl-diphosphoundecaprenol + UMP
-
 ?
2.7.8.36 UDP-6-hydroxybacillosamine + undecaprenyl phosphate
-
 Campylobacter jejuni ATCC 700819D 6-hydroxybacillosaminyl-diphosphoundecaprenol + UMP
-
 ?
2.7.8.36 UDP-bacillosamine + undecaprenyl phosphate UDP-N-acetylgalactosamine or UDP-N-acetylglucosamine not accepted as substrates Campylobacter jejuni bacillosaminyl-diphosphoundecaprenol + UMP
-
 ?
2.7.8.36 UDP-bacillosamine + undecaprenyl phosphate UDP-N-acetylgalactosamine or UDP-N-acetylglucosamine not accepted as substrates Campylobacter jejuni ATCC 700819D bacillosaminyl-diphosphoundecaprenol + UMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.36 bacillosamine 1-phosphoryl transferase
-
 Campylobacter jejuni
2.7.8.36 glycosyl-1-transferase
-
 Campylobacter jejuni
2.7.8.36 PglC
-
 Campylobacter jejuni