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Literature summary extracted from

  • Freel Meyers, C.L.; Oberthür, M.; Anderson, J.W.; Kahne, D.; Walsh, C.T.
    Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin (2003), Biochemistry, 42, 4179-4189.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.302 expression in Escherichia coli as a C-terminal His8 fusion protein Streptomyces niveus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.302 dTDP-beta-L-rhamnose
-
 Streptomyces niveus
2.4.1.302 novobiocic acid above 0.06 mM Streptomyces niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.302 0.0086
-
 dTDP-beta-L-noviose pH 6.0, 22°C Streptomyces niveus
2.4.1.302 0.032
-
 novobiocic acid pH 6.0, 22°C Streptomyces niveus
2.4.1.302 0.039
-
 cyclonovobiocic acid pH 6.0, 22°C Streptomyces niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.302 40000
-
 x * 40000, SDS-PAGE Streptomyces niveus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.302 dTDP-beta-L-noviose + novobiocic acid Streptomyces niveus the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin dTDP + demethyldecarbamoyl novobiocin i.e. demethyldecarbamoyl novobiocin ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.302 Streptomyces niveus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.302 C-terminal His8 fusion protein Streptomyces niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.302 dTDP-beta-L-noviose + novobiocic acid the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin Streptomyces niveus dTDP + demethyldecarbamoyl novobiocin i.e. demethyldecarbamoyl novobiocin ?
2.4.1.302 dTDP-beta-L-noviose + novobiocic acid no activity with TDP-L-rhamnose Streptomyces niveus dTDP + demethyldecarbamoyl novobiocin
-
 ?
2.4.1.302 dTDP-L-noviose + 4-nitrophenol kcat/Km is 208000fold lower compared to the value for the natural substrate novobiocic acid Streptomyces niveus dTDP + ?
-
 ?
2.4.1.302 dTDP-L-noviose + 7-hydroxy-4-methylcoumarin kcat/Km is 142000fold lower compared to the value for the natural substrate novobiocic acid Streptomyces niveus dTDP + ?
-
 ?
2.4.1.302 dTDP-L-noviose + 7-hydroxycoumarin kcat/Km is 172000fold lower compared to the value for the natural substrate novobiocic acid Streptomyces niveus dTDP + ?
-
 ?
2.4.1.302 dTDP-L-noviose + cyclonovobiocic acid kcat/KM is 30% compared to the value for the natural substrate novobiocic acid Streptomyces niveus dTDP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.302 ? x * 40000, SDS-PAGE Streptomyces niveus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.302 NovM
-
 Streptomyces niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.302 22
-
 assay at room temperature Streptomyces niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.302 118
-
 cyclonovobiocic acid pH 6.0, 22°C Streptomyces niveus
2.4.1.302 226
-
 dTDP-beta-L-noviose pH 6.0, 22°C Streptomyces niveus
2.4.1.302 313
-
 novobiocic acid pH 6.0, 22°C Streptomyces niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.302 6
-
 assay at Streptomyces niveus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.302 0.0835
-
 dTDP-beta-L-rhamnose pH 6.0, 22°C Streptomyces niveus

General Information

EC Number General Information Comment Organism
2.4.1.302 physiological function the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin Streptomyces niveus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.302 0.047
-
 4-nitrophenol pH 6.0, 22°C Streptomyces niveus
2.4.1.302 0.057
-
 7-hydroxycoumarin pH 6.0, 22°C Streptomyces niveus
2.4.1.302 0.069
-
 7-hydroxy-4-methylcoumarin pH 6.0, 22°C Streptomyces niveus
2.4.1.302 3026
-
 cyclonovobiocic acid pH 6.0, 22°C Streptomyces niveus
2.4.1.302 9781
-
 novobiocic acid pH 6.0, 22°C Streptomyces niveus