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Literature summary extracted from

  • Kendrew, S.G.; Katayama, K.; Deutsch, E.; Madduri, K.; Hutchinson, C.R.
    DnrD cyclase involved in the biosynthesis of doxorubicin: purification and characterization of the recombinant enzyme (1999), Biochemistry, 38, 4794-4799.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.23 overexpressed in Escherichia coli Streptomyces peucetius

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.23 additional information enzyme activity is not accelerated or significantly inhibited by divalent metal ions (Zn2+, Co2+, Fe2+, Cu2+, or Mn2+) or chelating reagents Streptomyces peucetius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.23 0.0521
-
 methyl aklanonate pH 7.0, 30°C Streptomyces peucetius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.23 additional information enzyme activity is not accelerated or significantly inhibited by divalent metal ions (Zn2+, Co2+, Fe2+, Cu2+, or Mn2+) or chelating reagents Streptomyces peucetius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.23 16000
-
 x * 16000, SDS-PAGE Streptomyces peucetius
5.5.1.23 16572
-
 x * 16572, electrospray MS analysis Streptomyces peucetius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.23 methyl aklanonate Streptomyces peucetius
-
 aklaviketone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.23 Streptomyces peucetius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.23
-
 Streptomyces peucetius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.23 14.3
-
 pH 7.0, 30°C Streptomyces peucetius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.23 methyl aklanonate
-
 Streptomyces peucetius aklaviketone
-
 ?
5.5.1.23 methyl aklanonate base-catalyzed mechanism Streptomyces peucetius aklaviketone
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.23 ? x * 16000, SDS-PAGE Streptomyces peucetius
5.5.1.23 ? x * 16572, electrospray MS analysis Streptomyces peucetius

Synonyms

EC Number Synonyms Comment Organism
5.5.1.23 AAMEcyclase
-
 Streptomyces peucetius
5.5.1.23 DnrD
-
 Streptomyces peucetius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.23 30
-
 assay at Streptomyces peucetius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.23 7
-
-
 Streptomyces peucetius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.23 6.5 9 pH 6.5: about 65% of maximal activity, 9.0: about 30% of maximal activity Streptomyces peucetius

General Information

EC Number General Information Comment Organism
5.5.1.23 physiological function the enzyme is involved in biosynthesis of aklaviketone, an intermediate in the biosynthetic pathway to daunorubicin and doxorubicin Streptomyces peucetius