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Literature summary extracted from

  • Bhatnagar, D.
    Enzymological evidence for separate pathways for aflatoxin B1 and B2 biosynthesis (1991), Biochemistry, 30, 4343-4350.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.117 0.0012
-
 8-O-methylsterigmatocystin in 50 mM phosphate buffer, pH 7.5, at 29°C Aspergillus parasiticus
1.14.14.117 0.0134
-
 8-O-methyldihydrosterigmatocystin in 50 mM phosphate buffer, pH 7.5, at 29°C Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus flavus
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 163
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus flavus SRRC 141
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus flavus preferred substrate aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus preferred substrate aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 163 preferred substrate aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus flavus SRRC 141 preferred substrate aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.117 Aspergillus flavus
-
-
-
1.14.14.117 Aspergillus flavus SRRC 141
-
-
-
1.14.14.117 Aspergillus parasiticus
-
-
-
1.14.14.117 Aspergillus parasiticus SRRC 163
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.117 ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration Aspergillus flavus
1.14.14.117 ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration Aspergillus parasiticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.117 0.23
-
 enzyme from supernatant, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C Aspergillus parasiticus
1.14.14.117 34.11
-
 after 148.3fold purification, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus flavus aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus SRRC 163 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus flavus SRRC 141 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 preferred substrate Aspergillus flavus aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 preferred substrate Aspergillus parasiticus aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 preferred substrate Aspergillus parasiticus SRRC 163 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 preferred substrate Aspergillus flavus SRRC 141 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.117 25 30
-
 Aspergillus flavus
1.14.14.117 25 30
-
 Aspergillus parasiticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.117 7.5
-
-
 Aspergillus flavus
1.14.14.117 7.5
-
-
 Aspergillus parasiticus