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Literature summary extracted from

  • Deng, L.L.; Alexander, A.A.; Lei, S.; Anderson, J.S.
    The cell wall teichuronic acid synthetase (TUAS) is an enzyme complex located in the cytoplasmic membrane of Micrococcus luteus (2010), Biochem. Res. Int., 2010, 395758.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.180 cell wall
-
 Micrococcus luteus 5618
-
2.4.1.180 plasma membrane
-
 Micrococcus luteus 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.180 52500
-
 4 * 54000 + 4 * 52500, TUAS complex, SDS-PAGE Micrococcus luteus
2.4.1.180 54000
-
 4 * 54000 + 4 * 52500, TUAS complex, SDS-PAGE Micrococcus luteus
2.4.1.180 440000
-
 TUAS complex Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.180 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.180 native TUAS complex partially by fractionation of Thesit-solubilized membrane fraction with ammonium sulfate Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.180 6
-
 TUAS embedded in the cell wall, pH 8.2, 25°C Micrococcus luteus
2.4.1.180 8.7
-
 TUAS embedded in the vesicle membrane, pH 8.2, 25°C Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.180 UDP-N-acetyl-alpha-D-mannosaminuronate + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Micrococcus luteus UDP + N-acetyl-beta-D-mannosaminuronyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.180 More when the eight subunits disassociate, the protein loses its conformation as well as the catalytic function for TUA biosynthesis Micrococcus luteus
2.4.1.180 octamer 4 * 54000 + 4 * 52500, TUAS complex, SDS-PAGE Micrococcus luteus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.180 ManNAcA-transferase
-
 Micrococcus luteus
2.4.1.180 teichuronic acid synthetase
-
 Micrococcus luteus
2.4.1.180 TUAS
-
 Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.180 25
-
 assay at Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.180 8.2
-
 assay at Micrococcus luteus

General Information

EC Number General Information Comment Organism
2.4.1.180 additional information the 440 kDa enzyme complex, named teichuronic acid synthetase, TUAS, is an octomer composed of two kinds of glycosyltransferases, glucosyltransferase, and ManNAcA-transferase, which is capable of catalyzing the transfer of disaccharide glycosyl residues containing both glucose and the N-acetylmannosaminuronic acid residues. TUAS is a bifunctional glycosyltransferase capable of transferring both the component sugars, glucose and ManNAcA, for the TUA polymer. The purified TUAS contains carotinoids and lipids. TUAS serves as a multitasking polysaccharide assembling station on the bacterial membrane Micrococcus luteus