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Literature summary extracted from

  • Vande Voorde, J.; Gago, F.; Vrancken, K.; Liekens, S.; Balzarini, J.
    Characterization of pyrimidine nucleoside phosphorylase of Mycoplasma hyorhinis: implications for the clinical efficacy of nucleoside analogues (2012), Biochem. J., 445, 113-123.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.2 medicine the cytostatic and antiviral activity of pyrimidine nucleoside analogues is markedly decreased by a Mycoplasma hyorhinis infection and the phosphorolytic activity of the mycoplasmas is responsible for this Mycoplasma hyorhinis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.2 expression in Escherichia coli Mycoplasma hyorhinis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.2 5-benzylacyclouridine
-
 Mycoplasma hyorhinis
2.4.2.2 5-chloro-6-(1-[2-iminopyrrolidinyl]methyl)uracil
-
 Mycoplasma hyorhinis
2.4.2.2 7-deazaxanthine
-
 Mycoplasma hyorhinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 0.047
-
 5-fluorouridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.069
-
 5-iodouridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.092
-
 uridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.144
-
 5-iododeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.169
-
 5-fluorodeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.388
-
 phosphate cosubstrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.473
-
 thymidine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.578
-
 deoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 0.797
-
 phosphate cosubstrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Mycoplasma hyorhinis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 5-bromodeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-bromouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-chlorodeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-chlorouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-fluorodeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-fluorouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-fluorouridine + phosphate
-
 Mycoplasma hyorhinis 5-fluorouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-iododeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-iodouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-iodouridine + phosphate
-
 Mycoplasma hyorhinis 5-iodouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-trifluoromethyl-2'-deoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-trifluoromethyluracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate
-
 Mycoplasma hyorhinis uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 additional information no substrate: cytidine, deoxycytidine, 2',2'-difluoro-2'-deoxycytidine, 2',2'-difluoro-2'-deoxyuridine, uracil arabinoside, thymine arabinoside, (E)-5-(2-bromovinyl)-deoxyuridine, azidothymidine, stavudine, 6-azauridine Mycoplasma hyorhinis ?
-
 ?
2.4.2.2 thymidine + phosphate
-
 Mycoplasma hyorhinis thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate prefered substrate Mycoplasma hyorhinis uracil + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.2 6.1
-
 5-fluorouridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 6.4
-
 5-iodouridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 8.5
-
 uridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 8.6
-
 phosphate cosubstrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 9.5
-
 5-iododeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 11.9
-
 5-fluorodeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 17.5
-
 phosphate cosubstrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 21.6
-
 thymidine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 24.6
-
 deoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.2 0.000003
-
 substrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis 5-benzylacyclouridine
2.4.2.2 0.000005
-
 substrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis 5-benzylacyclouridine
2.4.2.2 0.03
-
 substrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis 7-deazaxanthine
2.4.2.2 0.06
-
 substrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis 7-deazaxanthine
2.4.2.2 0.5
-
 substrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis 5-chloro-6-(1-[2-iminopyrrolidinyl]methyl)uracil
2.4.2.2 0.5
-
 substrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis 5-chloro-6-(1-[2-iminopyrrolidinyl]methyl)uracil

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.2 22
-
 phosphate cosubstrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 22
-
 phosphate cosubstrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 43
-
 deoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 46
-
 thymidine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 66
-
 5-iododeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 70
-
 5-fluorodeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 92
-
 uridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 93
-
 5-iodouridine pH 7.6, 37°C Mycoplasma hyorhinis
2.4.2.2 130
-
 5-fluorouridine pH 7.6, 37°C Mycoplasma hyorhinis