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Literature summary extracted from

  • Izumikawa, T.; Koike, T.; Kitagawa, H.
    Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1 (2012), Biochem. J., 441, 697-705.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.174 expression of ChGn-1 in mutant sog9 L cells Mus musculus
2.8.2.5 expressed in L cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.174 additional information construction of L-shRNA ChGn-1-1 and L-shRNA ChGn-1-2 transfected L cells, and analysis of chondroitin sulfate chain lengths. The silencing of the genes results in a 60-80% reduction in steady-state ChGn-1 mRNA and an 18-22% decrease in CS when compared with control L cells. Overexpression of ChGn-1 slightly increases CS levels in L cells Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.174 Mn2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.174 additional information Mus musculus in vitro chondroitin polymerization does not occur on the non-reducing terminal GalNAc-linkage pentasaccharide structure ?
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan Mus musculus
-
 UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan
-
 ?
2.8.2.5 3'-phosphoadenylyl sulfate + chondroitin Mus musculus
-
 adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.174 Mus musculus
-
-
-
2.8.2.5 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.5 heart
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.174 additional information in vitro chondroitin polymerization does not occur on the non-reducing terminal GalNAc-linkage pentasaccharide structure Mus musculus ?
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan
-
 Mus musculus UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan
-
 ?
2.8.2.5 3'-phosphoadenylyl sulfate + chondroitin
-
 Mus musculus adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.174 ChGn-1
-
 Mus musculus
2.4.1.174 chondroitin N-acetylgalactosaminyltransferase-1
-
 Mus musculus
2.8.2.5 C4ST-2
-
 Mus musculus
2.8.2.5 chondroitin 4-O-sulfotransferase-2
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.174 5.8
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.4.1.174 malfunction deficiency in chondroitin N-acetylgalactosaminyltransferase-1 reduces the numbers of chondroitin sulfate chains, leading to skeletal dysplasias in mice. Knockdown of ChGn-1 decreases chondroitin sulfate levels in L cells, chondroitin sulfate chain lengths in L-shRNAChGn-1-1, L-shRNA ChGn-1-2, and mock-transfected murine L cells, overview Mus musculus
2.4.1.174 physiological function ChGn-1 initiates chondroitin sulfate biosynthesis by transferring the first N-acetylgalactosamine to the tetrasaccharide in the protein linkage region of chondroitin sulfate, overview. c-2 efficiently transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of non-reducing terminal GalNAc-linkage residues, and the number of chondroitin chains is regulated by the expression levels of C4ST-2 and of ChGn-1. C4ST-2 plays a key role in regulating levels of chondroitin sulfate synthesized via ChGn-1, overview Mus musculus
2.8.2.5 metabolism the enzyme plays a key role in regulating levels of chondroitin sulfate synthesized via chondroitin N-acetylgalactosaminyltransferase-1 Mus musculus