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Literature summary extracted from

  • Yang, T.; Bar-Peled, M.
    Identification of a novel UDP-sugar pyrophosphorylase with a broad substrate specificity in Trypanosoma cruzi (2010), Biochem. J., 429, 533-543.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.64 expressed in Escherichia coli as a His-tagged fusion protein Trypanosoma cruzi
2.7.7.64 gene TcSloppy, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.64 EDTA
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 additional information
-
 additional information kinetics, overview Trypanosoma cruzi
2.7.7.64 0.026
-
 UDP-D-glucose pH 7.6, 37°C, recombinant enzyme Trypanosoma cruzi
2.7.7.64 0.026
-
 UDP-glucose reverse reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.0284
-
 UTP pH 7.6, 37°C, recombinant enzyme Trypanosoma cruzi
2.7.7.64 0.0284
-
 UTP forward reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.134
-
 diphosphate reverse reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.136
-
 diphosphate pH 7.6, 37°C, recombinant enzyme Trypanosoma cruzi
2.7.7.64 0.177
-
 alpha-D-glucose 1-phosphate pH 7.6, 37°C, recombinant enzyme Trypanosoma cruzi
2.7.7.64 0.177
-
 D-glucose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.177
-
 D-glucose 1-phosphate forward reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.818
-
 D-galactose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 1.76
-
 D-glucose 1-phosphate co-substrate: TTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 2.538
-
 D-xylose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 2.54
-
 TTP co-substrate: glucose 1-phosphate, pH 7.6, 37°C Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.64 Mg2+ Mg2+ or Mn2+ required Trypanosoma cruzi
2.7.7.64 Mg2+ the enzyme requires bivalent ions, Mg2+ or Mn2+, for its activity Trypanosoma cruzi
2.7.7.64 Mn2+ Mg2+ or Mn2+ required Trypanosoma cruzi
2.7.7.64 Mn2+ the enzyme requires bivalent ions, Mg2+ or Mn2+, for its activity Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.64 67000
-
 SDS-PAGE Trypanosoma cruzi
2.7.7.64 67000
-
 x * 67000, recombinant His-tagged enzyme, SDS-PAGE Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.64 additional information Trypanosoma cruzi the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars ?
-
 ?
2.7.7.64 TTP + alpha-D-glucose 1-phosphate Trypanosoma cruzi
-
 diphosphate + TDP-D-glucose
-
 ?
2.7.7.64 UTP + alpha-D-galactose 1-phosphate Trypanosoma cruzi
-
 diphosphate + UDP-D-galactose
-
 ?
2.7.7.64 UTP + alpha-D-glucose 1-phosphate Trypanosoma cruzi
-
 diphosphate + UDP-D-glucose
-
 r
2.7.7.64 UTP + alpha-D-glucuronate 1-phosphate Trypanosoma cruzi
-
 diphosphate + UDP-D-glucuronate
-
 ?
2.7.7.64 UTP + alpha-D-xylose 1-phosphate Trypanosoma cruzi
-
 diphosphate + UDP-D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Trypanosoma cruzi
-
-
-
2.7.7.64 Trypanosoma cruzi D3YBH2 gene Sloppy
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.64 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration Trypanosoma cruzi
2.7.7.64 using Ni-NTA chromatography Trypanosoma cruzi

Storage Stability

EC Number Storage Stability Organism
2.7.7.64 -80°C, flash-frozen in liquid nitrogen, several months Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 D-galactose 1-phosphate + UTP
-
 Trypanosoma cruzi UDP-galactose + diphosphate
-
 r
2.7.7.64 D-glucose 1-phosphate + TTP
-
 Trypanosoma cruzi TTP-glucose + diphosphate
-
 r
2.7.7.64 D-glucose 1-phosphate + UTP
-
 Trypanosoma cruzi UDP-glucose + diphosphate
-
 r
2.7.7.64 D-xylose 1-phosphate + UTP
-
 Trypanosoma cruzi UDP-xylose + diphosphate
-
 r
2.7.7.64 diphosphate + UDP-D-glucose
-
 Trypanosoma cruzi UTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.64 additional information the UDP-sugar pyrophosphorylase of Trypanosoma cruzi shows a broader substrate specificity, the nucleotidyltransferase is capable of transforming different types of sugar 1-phosphates and NTP into NDP-sugars Trypanosoma cruzi ?
-
 ?
2.7.7.64 TTP + alpha-D-glucose 1-phosphate
-
 Trypanosoma cruzi diphosphate + TDP-D-glucose
-
 ?
2.7.7.64 UTP + alpha-D-galactose 1-phosphate
-
 Trypanosoma cruzi diphosphate + UDP-D-galactose
-
 ?
2.7.7.64 UTP + alpha-D-glucose 1-phosphate
-
 Trypanosoma cruzi diphosphate + UDP-D-glucose
-
 r
2.7.7.64 UTP + alpha-D-glucuronate 1-phosphate
-
 Trypanosoma cruzi diphosphate + UDP-D-glucuronate
-
 ?
2.7.7.64 UTP + alpha-D-xylose 1-phosphate
-
 Trypanosoma cruzi diphosphate + UDP-D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.64 ? x * 67000, recombinant His-tagged enzyme, SDS-PAGE Trypanosoma cruzi
2.7.7.64 More real-time NMR analysis of TcSloppy, overview Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 TcSloppy
-
 Trypanosoma cruzi
2.7.7.64 UDP-sugar pyrophosphorylase
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.64 30 42
-
 Trypanosoma cruzi
2.7.7.64 37
-
 assay at Trypanosoma cruzi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.64 30 42 highly active between Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.64 0.085
-
 TTP co-substrate: glucose 1-phosphate, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.12
-
 D-glucose 1-phosphate co-substrate: TTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 8.6
-
 D-xylose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 26
-
 D-glucose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 35.2
-
 D-galactose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.64 6
-
 maximum activity in phosphate buffer Trypanosoma cruzi
2.7.7.64 6.5 8
-
 Trypanosoma cruzi
2.7.7.64 7.6
-
 assay at Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.64 6.5 8 highly active between Trypanosoma cruzi
2.7.7.64 7.5 7.8 maximum activity in Tris buffer Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
2.7.7.64 evolution phylogenetic analysis Trypanosoma cruzi
2.7.7.64 physiological function the parasite enzyme with such broad substrate specificities suggests an alternative pathway that might play an essential role for nucleotide-sugar biosynthesis and for the regulation of the NDP-sugar pool in the parasite Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.64 0.0334
-
 TTP co-substrate: glucose 1-phosphate, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 0.068
-
 D-glucose 1-phosphate co-substrate: TTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 34
-
 D-xylose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 40
-
 D-galactose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 150
-
 D-glucose 1-phosphate co-substrate: UTP, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 150
-
 D-glucose 1-phosphate forward reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 220
-
 diphosphate reverse reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 920
-
 UTP forward reaction, pH 7.6, 37°C Trypanosoma cruzi
2.7.7.64 1150
-
 UDP-glucose reverse reaction, pH 7.6, 37°C Trypanosoma cruzi