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Literature summary extracted from

  • Jackson, R.H.; Cole, J.A.; Cornish-Bowden, A.
    The steady state kinetics of the NADH-dependent nitrite reductase from Escherichia coli K12. The reduction of single-electron acceptors (1982), Biochem. J., 203, 505-510.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.15 NAD+ at 0.5 mM NAD+, the apparent maximum velocity is 2.3times higher for 0.1 mM cytochrome c as substrate than for 100 mM hydroxylamine Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.15 nitrite nitrite decrease the rate of NADH-dependent reduction of cytochrome c by 77% and that of Fe(CN)63- by 90% Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.15 0.0039
-
 cytochrome c apparent value, at pH 8.0 and 30°C Escherichia coli
1.7.1.15 0.011
-
 nitrite apparent value, at pH 8.0 and 30°C Escherichia coli
1.7.1.15 0.0488
-
 NADH with cytochrome c as cosubstrate, apparent value, at pH 8.0 and 30°C Escherichia coli
1.7.1.15 0.1
-
 K3Fe(CN)6 apparent value, at pH 8.0 and 30°C Escherichia coli
1.7.1.15 2.91
-
 hydroxylamine apparent value, at pH 8.0 and 30°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.15 nitrite + NADH + H+ Escherichia coli
-
 ammonia + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.15 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.15 2 ferricytochrome c + NADH
-
 Escherichia coli 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.7.1.15 ferricytochrome c + NADH + H+
-
 Escherichia coli ferrocytochrome c + NAD+
-
 r
1.7.1.15 hydroxylamine + NADH + H+
-
 Escherichia coli ?
-
 ?
1.7.1.15 K3Fe(CN)6 + NADH + H+
-
 Escherichia coli ?
-
 ?
1.7.1.15 nitrite + NADH + H+
-
 Escherichia coli ammonia + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.15 EC 1.6.6.4 formerly Escherichia coli
1.7.1.15 NADH-dependent nitrite reductase
-
 Escherichia coli
1.7.1.15 NADH:nitrite oxidoreductase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.15 NADH
-
 Escherichia coli