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Literature summary extracted from

  • Pettit, N.; Styslinger, T.; Mei, Z.; Han, W.; Zhao, G.; Wang, P.G.
    Characterization of WbiQ: An alpha1->2-fucosyltransferase from Escherichia coli O127:K63(B8), and synthesis of H-type 3 blood group antigen (2010), Biochem. Biophys. Res. Commun., 402, 190-195.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.69 additional information the enzyme exhibits full activity when 10 mM EDTA is present Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 the GST-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.69 inner membrane
-
Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 additional information the enzyme does not require the presence of a divalent metal to be enzymatically active Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.69 60000
-
2 * 60000, GST-tagged enzyme, SDS-PAGE Escherichia coli
2.4.1.69 61000
-
2 * 61000, calculated from amino acid sequence Escherichia coli
2.4.1.69 120000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Escherichia coli Q5J7C6
-
-
2.4.1.69 Escherichia coli O127:K63(B8) Q5J7C6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.69 glutathione Sepharose column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH
-
Escherichia coli GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH
-
Escherichia coli O127:K63(B8) GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe highest activity Escherichia coli GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe
-
?
2.4.1.69 GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe highest activity Escherichia coli O127:K63(B8) GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe
-
?
2.4.1.69 additional information the enzyme displays strict substrate specificity, displaying activity only towards acceptors that contain Gal-beta(1->3)-GalNAc-apha-OR linkages and does not recognize lactose, lactulose, Gal-alpha(1->3)-Gal-beta(1->4)Glc, alpha-GalGal-beta(1->4)-glucitol, and beta-D-Gal as acceptors Escherichia coli ?
-
?
2.4.1.69 additional information the enzyme displays strict substrate specificity, displaying activity only towards acceptors that contain Gal-beta(1->3)-GalNAc-apha-OR linkages and does not recognize lactose, lactulose, Gal-alpha(1->3)-Gal-beta(1->4)Glc, alpha-GalGal-beta(1->4)-glucitol, and beta-D-Gal as acceptors Escherichia coli O127:K63(B8) ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.69 homodimer 2 * 60000, GST-tagged enzyme, SDS-PAGE Escherichia coli
2.4.1.69 homodimer 2 * 61000, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.69 alpha1->2-fucosyltransferase
-
Escherichia coli
2.4.1.69 alpha1->2-FucT
-
Escherichia coli
2.4.1.69 WbiQ
-
Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 6.5 7.5
-
Escherichia coli