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Literature summary extracted from

  • Yi, W.; Yao, Q.; Zhang, Y.; Motari, E.; Lin, S.; Wang, P.G.
    The wbnH gene of Escherichia coli O86:H2 encodes an alpha-1,3-N-acetylgalactosaminyl transferase involved in the O-repeating unit biosynthesis (2006), Biochem. Biophys. Res. Commun., 344, 631-639.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.306 expression in Escherichia coli BL21(DE3) Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.306 additional information divalent metal cations are not required for enzyme catalysis Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.306 38000
-
x * 38000, SDS-PAGE Escherichia coli
2.4.1.306 38148
-
x * 38148, calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli 86:H2 the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.306 Escherichia coli
-
-
-
2.4.1.306 Escherichia coli 86:H2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.306
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.306 additional information the natural substrate N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol is difficult to isolate from natural bacterial sources due to the low abundance. The highly hydrophobic feature of the 55-carbon undecaprenyl chain makes it hard to handle Escherichia coli ?
-
?
2.4.1.306 additional information the natural substrate N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol is difficult to isolate from natural bacterial sources due to the low abundance. The highly hydrophobic feature of the 55-carbon undecaprenyl chain makes it hard to handle Escherichia coli 86:H2 ?
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3 95% of the activity with UDP-N-acetyl-D-galactosamine + D-GalNAc-alpha-diphospho-11-phenoxyundecanol Escherichia coli UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3 95% of the activity with UDP-N-acetyl-D-galactosamine + D-GalNAc-alpha-diphospho-11-phenoxyundecanol Escherichia coli 86:H2 UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol synthetic acceptor substrate. Diphosphate and hydrophobic lipid moiety are structural requirements for WbnH activity Escherichia coli UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol synthetic acceptor substrate. Diphosphate and hydrophobic lipid moiety are structural requirements for WbnH activity Escherichia coli 86:H2 UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria Escherichia coli UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
?
2.4.1.306 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria Escherichia coli 86:H2 UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.306 ? x * 38000, SDS-PAGE Escherichia coli
2.4.1.306 ? x * 38148, calculated from sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.306 WbnH
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.306 22
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.306 7
-
assay at, Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.306 Escherichia coli
-
-
6.4

General Information

EC Number General Information Comment Organism
2.4.1.306 physiological function the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria Escherichia coli