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Literature summary extracted from

  • Lee, M.J.; Chien-Liang, L.; Tsai, J.Y.; Sue, W.T.; Hsia, W.S.; Huang, H.
    Identification and biochemical characterization of a unique Mn2+-dependent UMP kinase from Helicobacter pylori (2010), Arch. Microbiol., 192, 739-746.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 GTP activates at 0.125 mM Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 expressed in Escherichia coli strain SG13009 Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 UTP 43% inhibition at 0.38 mM, 51.2% inhibition at 0.53 mM. About 21% of UMP kinase activity remains at 0.94-1.33 mM of UTP Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.054
-
 UMP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 0.125
-
 UMP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 0.304
-
 ATP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 0.4
-
 ATP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.22 Mg2+ 100% activity at 2 mM Helicobacter pylori
2.7.4.22 Mn2+ the enzyme prefers Mn2+ over Mg2+ Helicobacter pylori
2.7.4.22 additional information Co2+ and Ni2+ substitute poorly for Mg2+ and give limited enzyme activity, while Ca2+ and Zn2+ are unable to activate UMP kinase within 0-5 mM. UMP kinase activity of the HP0777 protein is completely abolished in the absence of divalent cations Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 27500
-
 x * 27500, His6-tagged enzyme, SDS-PAGE Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Helicobacter pylori P56106
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Helicobacter pylori ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 ? x * 27500, His6-tagged enzyme, SDS-PAGE Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 HP0777 protein
-
 Helicobacter pylori
2.7.4.22 uridine monophosphate kinase
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.22 117.9
-
 UMP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 189.6
-
 ATP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 436.2
-
 ATP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 545.2
-
 UMP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.22 7.4
-
 when Mn2+ serves as the cofactor, maximal enzyme activity is found at pH 7.4 Helicobacter pylori
2.7.4.22 9
-
 when Mg2+ serves as the cofactor, maximal enzyme activity is found at pH 9.0 Helicobacter pylori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.22 7.4
-
 the UMP kinase activity of the HP0777 protein is lost within a week when preserved at pH 9.0, but can be maintained for at least 2 weeks in 50 mM Tris-HCl, pH 7.4 Helicobacter pylori

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.4.22 0.45
-
 at pH 7.4 and 30°C Helicobacter pylori UTP

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.22 623.1
-
 ATP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 1250
-
 ATP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 2181
-
 UMP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
2.7.4.22 4362
-
 UMP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori