BRENDA - Enzyme Database

Tetrahydrofolate serves as a methyl acceptor in the demethylation of dimethylsulfoniopropionate in cell extracts of sulfate-reducing bacteria

Jansen, M.; Hansen, T.A.; Arch. Microbiol. 169, 84-87 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.269
O2
preincubation of the extract under air decreases the activity, 35% loss in 3 h
uncultured bacterium
2.1.1.269
Propyl iodide
0.5 mM, complete inhibition. In the light, the inhibition is far less strong
uncultured bacterium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.269
uncultured bacterium
-
-
-
2.1.1.269
uncultured bacterium WN
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.269
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
2.1.1.269
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium WN
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
2.1.1.269
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium
?
-
-
-
-
2.1.1.269
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium WN
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.269
28
-
assay at
uncultured bacterium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.269
7.2
-
assay at
uncultured bacterium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.269
tetrahydrofolate
methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate
uncultured bacterium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.269
tetrahydrofolate
methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate
uncultured bacterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.269
O2
preincubation of the extract under air decreases the activity, 35% loss in 3 h
uncultured bacterium
2.1.1.269
Propyl iodide
0.5 mM, complete inhibition. In the light, the inhibition is far less strong
uncultured bacterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.269
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
2.1.1.269
3-(S,S-dimethylsulfonio)propanoate + tetrahydrofolate
-
721447
uncultured bacterium WN
3-(methylthio)propanoate + 5-methyltetrahydrofolate
-
-
-
?
2.1.1.269
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium
?
-
-
-
-
2.1.1.269
additional information
no substrate: betaine. Reaction does not require ATP or reductive activation by titanium(III)-nitrilotriacetic acid
721447
uncultured bacterium WN
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.269
28
-
assay at
uncultured bacterium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.269
7.2
-
assay at
uncultured bacterium