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Literature summary extracted from

  • Bridges, C.C.; Krasnikov, B.F.; Joshee, L.; Pinto, J.T.; Hallen, A.; Li, J.; Zalups, R.K.; Cooper, A.J.
    New insights into the metabolism of organomercury compounds: mercury-containing cysteine S-conjugates are substrates of human glutamine transaminase K and potent inactivators of cystathionine gamma-lyase (2012), Arch. Biochem. Biophys., 517, 20-29.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.64
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.64 CH3-S-Hg-homocysteine 50% inhibition at 0.5 mM Homo sapiens
2.6.1.64 CH3Hg-S-Cys substrate and reversible inhibitor, 50% inhibition at 0.5 mM Homo sapiens
2.6.1.64 Cys-S-Hg-S-Cys substrate and reversible inhibitor, 37% inhibition at 0.5 mM Homo sapiens
2.6.1.64 homocysteine-S-Hg-S-homocysteine 67% inhibition at 1 mM Homo sapiens
4.4.1.1 4-chloromercuribenzoate
-
 Rattus norvegicus
4.4.1.1 CH3-Hg-S-Cys the cysteine S-conjugate of Hg2+ is a potent irreversible enzyme inhibitor Rattus norvegicus
4.4.1.1 Cys-S-Hg-S-Cys the cysteine S-conjugate of Hg2+ is a potent irreversible enzyme inhibitor Rattus norvegicus
4.4.1.1 HgCl2 a potent irreversible enzyme inhibitor, but HgCl2 is also a strong inactivator of cystathionine gamma-lyase Rattus norvegicus
4.4.1.1 Iodosobenzoate
-
 Rattus norvegicus
4.4.1.1 additional information extent to which inactivation occurs with the various mercury-containing compounds in descending order: Cys-S-Hg-S-Cys, HgCl2, CH3Hg-S-Cys Rattus norvegicus
4.4.1.1 N-ethylmaleimide
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.1 HgCl2 a potent irreversible enzyme inhibitor, but HgCl2 is also a strong inactivator of cystathionine gamma-lyase Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.1 L-cystathionine + H2O Rattus norvegicus
-
 2-oxobutyrate + L-cysteine + NH3
-
 ?
4.4.1.1 L-cystine + H2O Rattus norvegicus
-
 pyruvate + NH3 + L-thiocysteine
-
 ?
4.4.1.1 L-homoserine Rattus norvegicus the hydrolysis reaction is balanced by elimination of H2O, the net reaction does not include an H2O term 2-oxobutyrate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.64 Homo sapiens
-
-
-
4.4.1.1 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.1 24
-
 purified liver enzyme, pH 7.4, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.64 CH3Hg-S-Cys + phenylpyruvate
-
 Homo sapiens ? + L-phenylalanine
-
 ?
2.6.1.64 Cys-S-Hg-S-Cys + phenylpyruvate
-
 Homo sapiens ? + L-phenylalanine
-
 ?
2.6.1.64 L-cystathionine + phenylpyruvate
-
 Homo sapiens S-(2-oxo-2-carboxyethyl)homocysteine + L-phenylalanine
-
 ?
2.6.1.64 L-cysteine + phenylpyruvate
-
 Homo sapiens 3-mercapto-2-oxopropanoate + L-phenylalanine
-
 ?
2.6.1.64 L-methionine + phenylpyruvate
-
 Homo sapiens 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 ?
4.4.1.1 L-cystathionine + H2O
-
 Rattus norvegicus 2-oxobutyrate + L-cysteine + NH3
-
 ?
4.4.1.1 L-cystine + H2O
-
 Rattus norvegicus pyruvate + NH3 + L-thiocysteine
-
 ?
4.4.1.1 L-homoserine the hydrolysis reaction is balanced by elimination of H2O, the net reaction does not include an H2O term Rattus norvegicus 2-oxobutyrate + NH3
-
 ?
4.4.1.1 additional information no activity with L-methionine Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.1 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.1 7.4
-
 assay at Rattus norvegicus