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Literature summary extracted from

  • Manco, G.; Giosue, E.; DAuria, S.; Herman, P.; Carrea, G.; Rossi, M.
    Cloning, overexpression, and properties of a new thermophilic and thermostable esterase with sequence similarity to hormone-sensitive lipase subfamily from the archaeon Archaeoglobus fulgidus (2000), Arch. Biochem. Biophys., 373, 182-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 overexpression system in Escherichia coli Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 5,5'-dithiobis(2-nitrobenzoic)acid
-
 Archaeoglobus fulgidus
3.1.1.1 diethyldicarbonate 1.5 mM, 94% inhibition Archaeoglobus fulgidus
3.1.1.1 diisopropyl fluorophosphate irreversible Archaeoglobus fulgidus
3.1.1.1 phenylmethylsulfonyl fluoride 0.08 mM, 97% inhibition Archaeoglobus fulgidus
3.1.1.1 physostigmine 0.1 mM, 96% inhibition Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.011
-
 4-nitrophenyl hexanoate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 0.02
-
 4-nitrophenyl pentanoate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 0.021
-
 4-nitrophenyl butyrate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 0.106
-
 4-nitrophenyl propionate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 0.119
-
 4-nitrophenyl octanoate 70°C, pH 7.1 Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 33800
-
 1 * 33800, SDS-PAGE Archaeoglobus fulgidus
3.1.1.1 43000
-
 gel filtration Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Archaeoglobus fulgidus O28558
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 3000
-
 80°C, pH 7.1, substrate: 4-nitrophenyl hexanoate Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Archaeoglobus fulgidus 4-nitrophenol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 33800, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 AFEST
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 80
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 50 100 50°C: about 50% of maximal activity, 100°C: about 90% of maximal activity Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 58
-
 t1/2: 30 h Archaeoglobus fulgidus
3.1.1.1 70
-
 t1/2: 7.5 h Archaeoglobus fulgidus
3.1.1.1 85
-
 t1/2: 60 min Archaeoglobus fulgidus
3.1.1.1 90
-
 t1/2: 28 min Archaeoglobus fulgidus
3.1.1.1 95
-
 t1/2: 26 min Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 348
-
 4-nitrophenyl propionate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 680
-
 4-nitrophenyl butyrate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 770
-
 4-nitrophenyl octanoate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 866
-
 4-nitrophenyl pentanoate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 1014
-
 4-nitrophenyl hexanoate 70°C, pH 7.1 Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6.5 7.5
-
 Archaeoglobus fulgidus
3.1.1.1 7.1
-
 assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 3300
-
 4-nitrophenyl propionate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 6500
-
 4-nitrophenyl octanoate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 32400
-
 4-nitrophenyl butyrate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 43300
-
 4-nitrophenyl pentanoate 70°C, pH 7.1 Archaeoglobus fulgidus
3.1.1.1 92200
-
 4-nitrophenyl hexanoate 70°C, pH 7.1 Archaeoglobus fulgidus