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Literature summary extracted from

  • Ross, J.R.; Nam, K.H.; D'Auria, J.C.; Pichersky, E.
    S-Adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme involved in floral scent production and plant defense, represents a new class of plant methyltransferases (1999), Arch. Biochem. Biophys., 367, 9-16.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.274 SAMT cDNA expressed with pET-11a/pET-28 vector in Escherichia coli which synthesizes a functional non-tagged/His-tagged SAMT protein Clarkia breweri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.274 Ca2+ 5 mM, mild inhibitory effect, less than 35% reduced activity Clarkia breweri
2.1.1.274 Cu2+ 5 mM, strong inhibitory effect with 50–100% inhibition Clarkia breweri
2.1.1.274 Fe2+ 5 mM, strong inhibitory effect with 50–100% inhibition Clarkia breweri
2.1.1.274 Fe3+ 5 mM, strong inhibitory effect with 50–100% inhibition Clarkia breweri
2.1.1.274 Mg2+ 5 mM, mild inhibitory effect, less than 35% reduced activity Clarkia breweri
2.1.1.274 Mn2+ 5 mM, mild inhibitory effect, less than 35% reduced activity Clarkia breweri
2.1.1.274 additional information 5 mM Na+ and NH4+ have no effect on activity Clarkia breweri
2.1.1.274 Zn2+ 5 mM, strong inhibitory effect with 50–100% inhibition Clarkia breweri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.274 0.0017
-
 salicylate app. Km-value of His-tagged protein, pH 7.5, 20°C Clarkia breweri
2.1.1.274 0.009
-
 S-adenosyl-L-methionine app. Km-value of nontagged and His-tagged protein, pH 7.5, 20°C Clarkia breweri
2.1.1.274 0.024
-
 salicylate app. Km-value of nontagged protein, pH 7.5, 20°C Clarkia breweri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.274 K+ 5 mM, stimulates SAMT activity by a factor of 2 Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.274 40000
-
 monomer after Mono-Q chromatography Clarkia breweri
2.1.1.274 40300
-
 calculated from sequence Clarkia breweri
2.1.1.274 74500
-
 native protein Clarkia breweri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.274 S-adenosyl-L-methionine + salicylate Clarkia breweri
-
 S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.274 Clarkia breweri Q9SPV4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.274 by DE53 DEAE anion exchange, HAP and Mono-Q anion exchange chromatography Clarkia breweri

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.274 S-adenosyl-L-methionine + salicylate = S-adenosyl-L-homocysteine + methyl salicylate the enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase Clarkia breweri
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.274 petal highest SAMT activity compared to the levels of activity in all other floral parts or the vegetative tissue Clarkia breweri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.274 0.000228
-
 crude extract, pH 7.5, 20°C Clarkia breweri
2.1.1.274 0.00069
-
 DE-53 DEAE chromatography, pH 7.5, 20°C Clarkia breweri
2.1.1.274 0.00087
-
 HAP chromatography, pH 7.5, 20°C Clarkia breweri
2.1.1.274 0.02118
-
 Mono-Q chromatography, pH 7.5, 20°C Clarkia breweri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Clarkia breweri ?
-
 ?
2.1.1.274 additional information no activity with 4-hydroxybenzoic acid, vanillic acid, caffeic acid, p-coumaric acid and (+/-)jasmonic acid Clarkia breweri ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoic acid less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100% Clarkia breweri S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
 ?
2.1.1.274 S-adenosyl-L-methionine + cinnamic acid less than 2% relative activity at 1 mM methyl acceptor compared to activity with salicylate set at 100% Clarkia breweri S-adenosyl-L-homocysteine + methyl cinnamate
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Clarkia breweri S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.274 dimer 2 * 40300, calculated from sequence Clarkia breweri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.274 S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase
-
 Clarkia breweri
2.1.1.274 SAMT
-
 Clarkia breweri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.274 20 40 SAMT is 100% stable for 30 min at 20°C and 80% stable for 30 min at 30°C, but after 30 min incubation at 40°C it completely loses activity Clarkia breweri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.274 2.8
-
 salicylate nontagged protein, pH 7.5, 20°C Clarkia breweri
2.1.1.274 14
-
 salicylate His-tagged protein, pH 7.5, 20°C Clarkia breweri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.274 7.5
-
-
 Clarkia breweri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.274 6 9 at pH 6.0 activity is 80% of optimal activity, at pH 8.0 it is 75%, and at pH 9 it is less than 50% Clarkia breweri