BRENDA - Enzyme Database

The hyperthermophilic glycolytic enzyme enolase in the archaeon, Pyrococcus furiousus. Comparison with mesophilic enolases

Peak, M.; Peak, J.; Stevens, F.; Blamey, J.; Mai, X.; Zhou, Z.; Adams, M.; Arch. Biochem. Biophys. 313, 280-286 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
citrate
competitive
Pyrococcus furiosus
4.2.1.11
EDTA
1 mM
Pyrococcus furiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.4
-
2-phospho-D-glycerate
pH 8, 70°C
Pyrococcus furiosus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM
Pyrococcus furiosus
4.2.1.11
Mn2+
metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
2 * 46000
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Pyrococcus furiosus
the enzyme probably functions in sugar fermentation pathway
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Pyrococcus furiosus
Q9UWJ5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
13.8
-
pH 8, 70°C
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
721409
Pyrococcus furiosus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
the enzyme probably functions in sugar fermentation pathway
721409
Pyrococcus furiosus
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
2 * 46000
Pyrococcus furiosus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
90
-
above
Pyrococcus furiosus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.11
40
90
no activity at 25°C, activity increases from 40°C to 90°C, the highest assay temperature used
Pyrococcus furiosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
88
-
t1/2: 300 min
Pyrococcus furiosus
4.2.1.11
100
-
40 min, 50% inactivation. The half-life declines by a factor of about three in the presence of 700 mM KCl
Pyrococcus furiosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8.1
-
-
Pyrococcus furiosus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.13
-
citrate
pH 8, 70°C
Pyrococcus furiosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
citrate
competitive
Pyrococcus furiosus
4.2.1.11
EDTA
1 mM
Pyrococcus furiosus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.13
-
citrate
pH 8, 70°C
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.4
-
2-phospho-D-glycerate
pH 8, 70°C
Pyrococcus furiosus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM
Pyrococcus furiosus
4.2.1.11
Mn2+
metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
2 * 46000
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Pyrococcus furiosus
the enzyme probably functions in sugar fermentation pathway
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
13.8
-
pH 8, 70°C
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
721409
Pyrococcus furiosus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
the enzyme probably functions in sugar fermentation pathway
721409
Pyrococcus furiosus
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
2 * 46000
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
90
-
above
Pyrococcus furiosus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.11
40
90
no activity at 25°C, activity increases from 40°C to 90°C, the highest assay temperature used
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
88
-
t1/2: 300 min
Pyrococcus furiosus
4.2.1.11
100
-
40 min, 50% inactivation. The half-life declines by a factor of about three in the presence of 700 mM KCl
Pyrococcus furiosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8.1
-
-
Pyrococcus furiosus
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
the enzyme probably functions in sugar fermentation pathway
Pyrococcus furiosus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
the enzyme probably functions in sugar fermentation pathway
Pyrococcus furiosus