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Literature summary extracted from

  • Peak, M.; Peak, J.; Stevens, F.; Blamey, J.; Mai, X.; Zhou, Z.; Adams, M.
    The hyperthermophilic glycolytic enzyme enolase in the archaeon, Pyrococcus furiousus. Comparison with mesophilic enolases (1994), Arch. Biochem. Biophys., 313, 280-286.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 citrate competitive Pyrococcus furiosus
4.2.1.11 EDTA 1 mM Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.4
-
 2-phospho-D-glycerate pH 8, 70°C Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM Pyrococcus furiosus
4.2.1.11 Mn2+ metal ion required, equally active in presence of Mg2+ and Mn2+, 1 mM Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 46000
-
 2 * 46000 Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Pyrococcus furiosus the enzyme probably functions in sugar fermentation pathway phosphoenolpyruvate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Pyrococcus furiosus Q9UWJ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 13.8
-
 pH 8, 70°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Pyrococcus furiosus phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate the enzyme probably functions in sugar fermentation pathway Pyrococcus furiosus phosphoenolpyruvate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.11 homodimer 2 * 46000 Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.11 90
-
 above Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.11 40 90 no activity at 25°C, activity increases from 40°C to 90°C, the highest assay temperature used Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.11 88
-
 t1/2: 300 min Pyrococcus furiosus
4.2.1.11 100
-
 40 min, 50% inactivation. The half-life declines by a factor of about three in the presence of 700 mM KCl Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 8.1
-
-
 Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.11 0.13
-
 citrate pH 8, 70°C Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
4.2.1.11 physiological function the enzyme probably functions in sugar fermentation pathway Pyrococcus furiosus