EC Number | General Stability | Organism |
---|---|---|
2.7.4.27 | stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue | Zea mays |
2.7.11.32 | stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.27 | ADP | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
2.7.4.27 | ADP-beta-S | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
2.7.4.27 | AMP | competitive to phosphate | Zea mays | |
2.7.4.27 | CDP | - |
Zea mays | |
2.7.4.27 | Cibacron blue | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
2.7.4.27 | diphosphate | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
2.7.4.27 | GDP | - |
Zea mays | |
2.7.4.27 | pyruvate,Pi dikinase phosphorylated at a His residue | competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) | Zea mays | |
2.7.11.32 | ADP-beta-S | competitive to ADP | Zea mays | |
2.7.11.32 | pyruvate,Pi dikinase phosphorylated at a Thr residue | competitive to ADP; competitive to substrate pyruvate,Pi dikinase | Zea mays | |
2.7.11.32 | [pyruvate,Pi dikinase]phosphate | competitive to substrate pyruvate,Pi dikinase; noncompetitive to ADP | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.27 | 0.0007 | - |
[pyruvate,Pi dikinase]phosphate | phosphorylated at a Thr residue, pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.4.27 | 0.65 | - |
phosphate | pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.11.32 | 0.0012 | - |
pyruvate,Pi dikinase | pyruvate,Pi dikinase phosphorylated at a His residue, pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.11.32 | 0.052 | - |
ADP | pH 8.3, temperature not specified in the publication | Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.27 | Mg2+ | - |
Zea mays | |
2.7.11.32 | Mg2+ | - |
Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.27 | 45000 | - |
x * 45000, SDS-PAGE | Zea mays |
2.7.4.27 | 90000 | - |
pH 7.5, gel filtration | Zea mays |
2.7.4.27 | 180000 | - |
pH 8.3, gel filtration | Zea mays |
2.7.11.32 | 45000 | - |
x * 45000, SDS-PAGE | Zea mays |
2.7.11.32 | 90000 | - |
pH 7.5, gel filtration | Zea mays |
2.7.11.32 | 180000 | - |
pH 8.3, gel filtration | Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.27 | Zea mays | - |
cv. Dekalb XL81 | - |
2.7.11.32 | Zea mays | - |
cv. Dekalb XL81 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.27 | ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration | Zea mays |
2.7.11.32 | ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.27 | [pyruvate,Pi dikinase]phosphate + phosphate | phosphorylated at a Thr residue, inactive, additionally catalytically phosphorylated substrate (at His residue) is a much poorer substrate | Zea mays | pyruvate,Pi dikinase + diphosphate | active | ? | |
2.7.11.32 | pyruvate,Pi dikinase + ADP | substrate pyruvate,Pi dikinase is phosphorylated at a His residue and active; CDP shows 40% activity, GDP 35% activity and UDP 0% activity in comparison with ADP | Zea mays | [pyruvate,Pi dikinase]phosphate + AMP | phosphorylated at a Thr residue, inactive | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.27 | ? | x * 45000, SDS-PAGE | Zea mays |
2.7.11.32 | ? | x * 45000, SDS-PAGE | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.27 | pyruvate,Pi dikinase regulatory protein | bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase | Zea mays |
2.7.11.32 | pyruvate,Pi dikinase regulatory protein | bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.27 | 8.1 | - |
broad optimum pH 7.8-8.4, 68% activity at pH 7.2 | Zea mays |
2.7.11.32 | 8.1 | - |
broad optimum pH 7.8-8.4, 68% activity at pH 7.2 | Zea mays |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.27 | 0.005 | - |
Cibacron blue | pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.4.27 | 0.08 | - |
ADP-beta-S | Ki-value below 0.08 mM, pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.4.27 | 0.085 | - |
ADP | pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.4.27 | 0.16 | - |
diphosphate | pH 8.3, temperature not specified in the publication | Zea mays | |
2.7.4.27 | 0.4 | - |
AMP | pH 8.3, temperature not specified in the publication | Zea mays |