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Literature summary extracted from

  • Burnell, J.; Hatch, M.
    Regulation of C4 photosynthesis: Purification and properties of the protein catalyzing ADP-mediated inactivation and Pi-mediated activation of pyruvate,Pi dikinase (1985), Arch. Biochem. Biophys., 237, 490-503.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.27 stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue Zea mays
2.7.11.32 stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.27 ADP competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) Zea mays
2.7.4.27 ADP-beta-S competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) Zea mays
2.7.4.27 AMP competitive to phosphate Zea mays
2.7.4.27 CDP
-
Zea mays
2.7.4.27 Cibacron blue competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) Zea mays
2.7.4.27 diphosphate competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) Zea mays
2.7.4.27 GDP
-
Zea mays
2.7.4.27 pyruvate,Pi dikinase phosphorylated at a His residue competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue) Zea mays
2.7.11.32 ADP-beta-S competitive to ADP Zea mays
2.7.11.32 pyruvate,Pi dikinase phosphorylated at a Thr residue competitive to ADP; competitive to substrate pyruvate,Pi dikinase Zea mays
2.7.11.32 [pyruvate,Pi dikinase]phosphate competitive to substrate pyruvate,Pi dikinase; noncompetitive to ADP Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.27 0.0007
-
[pyruvate,Pi dikinase]phosphate phosphorylated at a Thr residue, pH 8.3, temperature not specified in the publication Zea mays
2.7.4.27 0.65
-
phosphate pH 8.3, temperature not specified in the publication Zea mays
2.7.11.32 0.0012
-
pyruvate,Pi dikinase pyruvate,Pi dikinase phosphorylated at a His residue, pH 8.3, temperature not specified in the publication Zea mays
2.7.11.32 0.052
-
ADP pH 8.3, temperature not specified in the publication Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.27 Mg2+
-
Zea mays
2.7.11.32 Mg2+
-
Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.27 45000
-
x * 45000, SDS-PAGE Zea mays
2.7.4.27 90000
-
pH 7.5, gel filtration Zea mays
2.7.4.27 180000
-
pH 8.3, gel filtration Zea mays
2.7.11.32 45000
-
x * 45000, SDS-PAGE Zea mays
2.7.11.32 90000
-
pH 7.5, gel filtration Zea mays
2.7.11.32 180000
-
pH 8.3, gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.27 Zea mays
-
cv. Dekalb XL81
-
2.7.11.32 Zea mays
-
cv. Dekalb XL81
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.27 ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration Zea mays
2.7.11.32 ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.27 [pyruvate,Pi dikinase]phosphate + phosphate phosphorylated at a Thr residue, inactive, additionally catalytically phosphorylated substrate (at His residue) is a much poorer substrate Zea mays pyruvate,Pi dikinase + diphosphate active ?
2.7.11.32 pyruvate,Pi dikinase + ADP substrate pyruvate,Pi dikinase is phosphorylated at a His residue and active; CDP shows 40% activity, GDP 35% activity and UDP 0% activity in comparison with ADP Zea mays [pyruvate,Pi dikinase]phosphate + AMP phosphorylated at a Thr residue, inactive ?

Subunits

EC Number Subunits Comment Organism
2.7.4.27 ? x * 45000, SDS-PAGE Zea mays
2.7.11.32 ? x * 45000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.4.27 pyruvate,Pi dikinase regulatory protein bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase Zea mays
2.7.11.32 pyruvate,Pi dikinase regulatory protein bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.27 8.1
-
broad optimum pH 7.8-8.4, 68% activity at pH 7.2 Zea mays
2.7.11.32 8.1
-
broad optimum pH 7.8-8.4, 68% activity at pH 7.2 Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.27 0.005
-
Cibacron blue pH 8.3, temperature not specified in the publication Zea mays
2.7.4.27 0.08
-
ADP-beta-S Ki-value below 0.08 mM, pH 8.3, temperature not specified in the publication Zea mays
2.7.4.27 0.085
-
ADP pH 8.3, temperature not specified in the publication Zea mays
2.7.4.27 0.16
-
diphosphate pH 8.3, temperature not specified in the publication Zea mays
2.7.4.27 0.4
-
AMP pH 8.3, temperature not specified in the publication Zea mays