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Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides. Interaction of the enzyme with coenzymes and coenzyme analogs

Grove, T.H.; Ishaque, A.; Levy, H.R.; Arch. Biochem. Biophys. 17, 307-316 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.363
2',5'-ADP
NADP+-competitive and NAD+-noncompetitive inhibition
Leuconostoc mesenteroides
1.1.1.363
2'-AMP
-
Leuconostoc mesenteroides
1.1.1.363
5'-AMP
-
Leuconostoc mesenteroides
1.1.1.363
adenosine
-
Leuconostoc mesenteroides
1.1.1.363
ADP
-
Leuconostoc mesenteroides
1.1.1.363
ADP-ribose
-
Leuconostoc mesenteroides
1.1.1.363
ATP
-
Leuconostoc mesenteroides
1.1.1.363
D-glucose 1-phosphate
a substrate-competitive inhibitor, that lowers the dissociation constant and maximum fluorescence quenching for NAD+ but not for NADP+
Leuconostoc mesenteroides
1.1.1.363
N'-methylnicotinamide
-
Leuconostoc mesenteroides
1.1.1.363
nicotinamide
-
Leuconostoc mesenteroides
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.363
D-glucose 6-phosphate + NAD+
Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
?
1.1.1.363
D-glucose 6-phosphate + NADP+
Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.363
Leuconostoc mesenteroides
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.363
-
Leuconostoc mesenteroides
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.363
D-glucose 6-phosphate + NAD+
-
721400
Leuconostoc mesenteroides
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
1.1.1.363
D-glucose 6-phosphate + NADP+
-
721400
Leuconostoc mesenteroides
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.363
NAD+
the maximum quenching of protein fluorescence is 50% for NAD+. The dissociation constant for NAD+ is 2.5 mM
Leuconostoc mesenteroides
1.1.1.363
NADP+
the maximum quenching of protein fluorescence is 5% for NADP+. The dissociation constant for NADP+ is 0.003 mM
Leuconostoc mesenteroides
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.363
0.12
-
2',5'-ADP
pH 7.8, 25°C, substrate: NADP+
Leuconostoc mesenteroides
1.1.1.363
0.52
-
ADP-ribose
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
1.3
-
2'-AMP
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
1.5
-
ATP
pH 7.8, 25°C, substrate: NADP+
Leuconostoc mesenteroides
1.1.1.363
2
-
5'-AMP
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
3.1
-
ADP
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
36
-
adenosine
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
95
-
N'-methylnicotinamide
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
105
-
nicotinamide
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.363
NAD+
the maximum quenching of protein fluorescence is 50% for NAD+. The dissociation constant for NAD+ is 2.5 mM
Leuconostoc mesenteroides
1.1.1.363
NADP+
the maximum quenching of protein fluorescence is 5% for NADP+. The dissociation constant for NADP+ is 0.003 mM
Leuconostoc mesenteroides
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.363
2',5'-ADP
NADP+-competitive and NAD+-noncompetitive inhibition
Leuconostoc mesenteroides
1.1.1.363
2'-AMP
-
Leuconostoc mesenteroides
1.1.1.363
5'-AMP
-
Leuconostoc mesenteroides
1.1.1.363
adenosine
-
Leuconostoc mesenteroides
1.1.1.363
ADP
-
Leuconostoc mesenteroides
1.1.1.363
ADP-ribose
-
Leuconostoc mesenteroides
1.1.1.363
ATP
-
Leuconostoc mesenteroides
1.1.1.363
D-glucose 1-phosphate
a substrate-competitive inhibitor, that lowers the dissociation constant and maximum fluorescence quenching for NAD+ but not for NADP+
Leuconostoc mesenteroides
1.1.1.363
N'-methylnicotinamide
-
Leuconostoc mesenteroides
1.1.1.363
nicotinamide
-
Leuconostoc mesenteroides
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.363
0.12
-
2',5'-ADP
pH 7.8, 25°C, substrate: NADP+
Leuconostoc mesenteroides
1.1.1.363
0.52
-
ADP-ribose
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
1.3
-
2'-AMP
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
1.5
-
ATP
pH 7.8, 25°C, substrate: NADP+
Leuconostoc mesenteroides
1.1.1.363
2
-
5'-AMP
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
3.1
-
ADP
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
36
-
adenosine
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
95
-
N'-methylnicotinamide
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
1.1.1.363
105
-
nicotinamide
pH 7.8, 25°C, substrate: NAD+
Leuconostoc mesenteroides
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.363
D-glucose 6-phosphate + NAD+
Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
?
1.1.1.363
D-glucose 6-phosphate + NADP+
Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.363
-
Leuconostoc mesenteroides
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.363
D-glucose 6-phosphate + NAD+
-
721400
Leuconostoc mesenteroides
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
-
?
1.1.1.363
D-glucose 6-phosphate + NADP+
-
721400
Leuconostoc mesenteroides
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?