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Literature summary extracted from

  • Yin, S.; Yu, X.; Wang, Q.; Liu, X.Q.; Li, S.M.
    Identification of a brevianamide F reverse prenyltransferase BrePT from Aspergillus versicolor with a broad substrate specificity towards tryptophan-containing cyclic dipeptides (2013), Appl. Microbiol. Biotechnol., 97, 1649-1660.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.109 overexpression in Escherichia coli Aspergillus versicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.109 0.032
-
brevianamide F pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.032
-
brevianamide F 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.082
-
cyclo-L-Trp-D-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.082
-
cyclo-L-Trp-D-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.094
-
cyclo-L-Trp-L-Phe pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.094
-
cyclo-L-Trp-L-Phe 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.098
-
dimethylallyl diphosphate pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.098
-
dimethylallyl diphosphate 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.106
-
cyclo-L-Trp-L-Leu pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.106
-
cyclo-L-Trp-L-Leu 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.139
-
cyclo-D-Trp-L-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.214
-
cyclo-L-Trp-L-Tyr pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.214
-
cyclo-L-Trp-L-Tyr 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.709
-
cyclo-D-Trp-D-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.709
-
cyclo-D-Trp-D-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.793
-
cyclo-L-Trp-D-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.793
-
cyclo-L-Trp-D-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.942
-
cyclo-L-Trp-L-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.942
-
cyclo-L-Trp-L-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 1.3
-
cyclo-L-Trp-Gly pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 1.3
-
cyclo-L-Trp-Gly 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 1.318
-
cyclo-D-Trp-D-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 1.318
-
cyclo-D-Trp-D-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 1.318
-
cyclo-D-Trp-L-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 2.906
-
cyclo-D-Trp-L-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 2.906
-
cyclo-D-Trp-L-Pro 37°C, pH 7.5 Aspergillus versicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.109 45000
-
x * 45000, SDS-PAGE Aspergillus versicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.109 dimethylallyl diphosphate + brevianamide F Aspergillus versicolor
-
diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F Aspergillus versicolor NRRL 573
-
diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F Aspergillus versicolor NRRL573
-
diphosphate + deoxybrevianamide E
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.109 Aspergillus versicolor I4AY86
-
-
2.5.1.109 Aspergillus versicolor NRRL 573 I4AY86
-
-
2.5.1.109 Aspergillus versicolor NRRL573 I4AY86
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.109
-
Aspergillus versicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.109 dimethylallyl diphosphate + brevianamide F
-
Aspergillus versicolor diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate Aspergillus versicolor diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F i.e. cyclo-L-Trp-L-Pro Aspergillus versicolor diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F
-
Aspergillus versicolor NRRL 573 diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate Aspergillus versicolor NRRL 573 diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F i.e. cyclo-L-Trp-L-Pro Aspergillus versicolor NRRL 573 diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F
-
Aspergillus versicolor NRRL573 diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate Aspergillus versicolor NRRL573 diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + brevianamide F i.e. cyclo-L-Trp-L-Pro Aspergillus versicolor NRRL573 diphosphate + deoxybrevianamide E
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
Aspergillus versicolor diphosphate +
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
Aspergillus versicolor NRRL 573 diphosphate +
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
Aspergillus versicolor NRRL573 diphosphate +
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
Aspergillus versicolor diphosphate + ?
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-D-Trp]-D-Pro
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-D-Trp]-L-Ala
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-D-Trp]-L-Pro
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-L-Trp]-D-Ala
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-L-Trp]-D-Pro
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-L-Trp]-Gly
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-L-Trp]-L-Ala
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
Aspergillus versicolor diphosphate + ?
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-L-Trp]-L-Leu
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Phe
-
Aspergillus versicolor diphosphate +
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Phe
-
Aspergillus versicolor diphosphate + ?
-
?
2.5.1.109 dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
Aspergillus versicolor diphosphate + cyclo-[2-(2-methylbut-3-en-2-yl)-L-Trp]-L-Tyr
-
?
2.5.1.109 additional information enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus Aspergillus versicolor ?
-
?
2.5.1.109 additional information enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus Aspergillus versicolor NRRL 573 ?
-
?
2.5.1.109 additional information enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus Aspergillus versicolor NRRL573 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.109 ? x * 45000, SDS-PAGE Aspergillus versicolor

Synonyms

EC Number Synonyms Comment Organism
2.5.1.109 BrePT
-
Aspergillus versicolor
2.5.1.109 brevianamide F reverse prenyltransferase
-
Aspergillus versicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.109 0.003
-
cyclo-D-Trp-L-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.003
-
cyclo-D-Trp-L-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.004
-
cyclo-L-Trp-L-Phe pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.004
-
cyclo-L-Trp-L-Phe 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.006
-
cyclo-L-Trp-L-Tyr pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.006
-
cyclo-L-Trp-L-Tyr 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.011
-
cyclo-D-Trp-D-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.011
-
cyclo-D-Trp-D-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.013
-
cyclo-L-Trp-D-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.013
-
cyclo-L-Trp-D-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.014
-
cyclo-L-Trp-L-Leu pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.014
-
cyclo-L-Trp-L-Leu 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.027
-
cyclo-D-Trp-L-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.027
-
cyclo-D-Trp-L-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.032
-
cyclo-L-Trp-Gly pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.032
-
cyclo-L-Trp-Gly 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.035
-
cyclo-L-Trp-L-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.035
-
cyclo-L-Trp-L-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.095
-
cyclo-D-Trp-D-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.095
-
cyclo-D-Trp-D-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.15
-
cyclo-L-Trp-D-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.15
-
cyclo-L-Trp-D-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.276
-
brevianamide F pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.276
-
brevianamide F 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.489
-
dimethylallyl diphosphate 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.49
-
dimethylallyl diphosphate pH 7.5, 37°C Aspergillus versicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.109 0.009
-
cyclo-D-Trp-L-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.009
-
cyclo-D-Trp-D-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.009
-
cyclo-D-Trp-L-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.009
-
cyclo-D-Trp-D-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.017
-
cyclo-L-Trp-D-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.017
-
cyclo-L-Trp-D-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.021
-
cyclo-D-Trp-L-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.021
-
cyclo-D-Trp-L-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.025
-
cyclo-L-Trp-Gly pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.025
-
cyclo-L-Trp-Gly 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.027
-
cyclo-L-Trp-L-Tyr pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.027
-
cyclo-L-Trp-L-Tyr 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.037
-
cyclo-L-Trp-L-Ala pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.037
-
cyclo-L-Trp-L-Ala 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.04
-
cyclo-L-Trp-L-Phe pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.04
-
cyclo-L-Trp-L-Phe 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.13
-
cyclo-D-Trp-D-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.13
-
cyclo-D-Trp-D-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 0.134
-
cyclo-L-Trp-L-Leu pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 0.134
-
cyclo-L-Trp-L-Leu 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 1.8
-
cyclo-L-Trp-D-Pro pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 1.83
-
cyclo-L-Trp-D-Pro 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 4.99
-
dimethylallyl diphosphate pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 4.99
-
dimethylallyl diphosphate 37°C, pH 7.5 Aspergillus versicolor
2.5.1.109 8.6
-
brevianamide F pH 7.5, 37°C Aspergillus versicolor
2.5.1.109 8.64
-
brevianamide F 37°C, pH 7.5 Aspergillus versicolor