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Literature summary extracted from

  • Bogicevic, B.; Berthoud, H.; Portmann, R.; Meile, L.; Irmler, S.
    CysK from Lactobacillus casei encodes a protein with O-acetylserine sulfhydrylase and cysteine desulfurization activity (2012), Appl. Microbiol. Biotechnol., 94, 1209-1220.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expression in Escherichia coli Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 additional information presence of 4% NaCl is not inhibitory Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.6
-
 O-acetyl-L-Ser pH 7.4, 37°C Lacticaseibacillus casei
2.5.1.47 0.7
-
 dithiothreitol pH 7.4, 37°C Lacticaseibacillus casei
2.5.1.47 0.7
-
 L-Cys pH 7.4, 37°C Lacticaseibacillus casei
2.5.1.47 6.7
-
 H2S pH 7.4, 37°C Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 32000
-
 PAGE Lacticaseibacillus casei
2.5.1.47 34900
-
 1 * 32000, SDS-PAGE, 1 * 34900, calculated Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Lacticaseibacillus casei E5LPF4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 L-Cys + dithiothreitol
-
 Lacticaseibacillus casei beta-cyanoalanine + H2S
-
 ?
2.5.1.47 additional information no substrates: serine, phosphoserine, O-succinylhomoserine, thiosulfate Lacticaseibacillus casei ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Lacticaseibacillus casei L-Cys + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 monomer 1 * 32000, SDS-PAGE, 1 * 34900, calculated Lacticaseibacillus casei

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 cysK
-
 Lacticaseibacillus casei

Expression

EC Number Organism Comment Expression
2.5.1.47 Lacticaseibacillus casei expression is not regulated by presence of cysteine additional information

General Information

EC Number General Information Comment Organism
2.5.1.47 physiological function enzyme is able to complement the cysteine auxotrophy of an Escherichia coli cysMK mutant Lacticaseibacillus casei