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Literature summary extracted from

  • Tian, Y.S.; Xu, J.; Xiong, A.S.; Zhao, W.; Gao, F.; Fu, X.Y.; Peng, R.H.; Yao, Q.H.
    Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis (2012), Appl. Microbiol. Biotechnol., 93, 241-250.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.19 agriculture transgenic Arabidopsis thaliana plants expressing the enzyme from Halothermothrix orenii are more resistant to glyphosate exposure than transgenic plants expressing the Escherichia coli enzyme and control plants Halothermothrix orenii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression in Escherichia coli Halothermothrix orenii

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 T355S mutation results in a catalytically more efficient glyphosate-resistant enzyme Halothermothrix orenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 glyphosate
-
 Halothermothrix orenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.241
-
 3-phosphoshikimate mutant T355S, pH 7.0, 28°C Halothermothrix orenii
2.5.1.19 0.286
-
 3-phosphoshikimate wild-type, pH 7.0, 28°C Halothermothrix orenii
2.5.1.19 0.292
-
 phosphoenolpyruvate mutant T355S, pH 7.0, 28°C Halothermothrix orenii
2.5.1.19 0.316
-
 phosphoenolpyruvate wild-type, pH 7.0, 28°C Halothermothrix orenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 46020
-
 x * 46020, calculated Halothermothrix orenii

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Halothermothrix orenii B8CWX4
-
-
2.5.1.19 Halothermothrix orenii H 168 B8CWX4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Halothermothrix orenii phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Halothermothrix orenii H 168 phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 ? x * 46020, calculated Halothermothrix orenii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.0446
-
 glyphosate wild-type, pH 7.0, 28°C Halothermothrix orenii
2.5.1.19 0.0512
-
 glyphosate mutant T355S, pH 7.0, 28°C Halothermothrix orenii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.19 0.328
-
 wild-type, pH 7.0, 28°C Halothermothrix orenii glyphosate
2.5.1.19 0.398
-
 mutant T355S, pH 7.0, 28°C Halothermothrix orenii glyphosate