EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.248 | overexpression of CITase in Bacillus circulans strain G22-10, and, with the alpha-amylase promoter (PamyQ) and amyQ signal sequence of Bacillus amyloliquefaciens, in a protease-deficient Bacillus subtilis strain 168 as extracellular protein. The Bacillus subtilis host-vector system allows production of cycloisomaltooligosaccharides by direct fermentation of dextran along with high CITase production, which is not possible in Bacillus circulans G22-10 due to growth inhibition by dextran at high concentrations and limited production of CITase, stable expression up to 48 h | Niallia circulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.248 | 103000 | - |
recombinant CITAse, gel filtration | Niallia circulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.248 | Niallia circulans | P94286 | - |
- |
2.4.1.248 | Niallia circulans T-3040 | P94286 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.248 | recombinant extracellular CITase from protease-deficient Bacillus subtilis strain 168 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration and another step of anion exchange chromatography, recombinant extracellular CITase from Bacillus circulans G22-10 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Niallia circulans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.248 | 14.2 | - |
recombinant CITase from Bacillus circulans strain G22-10, pH 5.5, 40°C | Niallia circulans |
2.4.1.248 | 14.6 | - |
recombinant CITase from Bacillus subtilis strain 168 DELTAaprE DELTAnprE, pH 5.5, 40°C | Niallia circulans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.248 | CITase | - |
Niallia circulans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.248 | 40 | - |
assay at | Niallia circulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.248 | 5.5 | - |
assay at | Niallia circulans |