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Literature summary extracted from

  • Kawabata, Y.; Kimura, K.; Funane, K.
    Extracellular production of cycloisomaltooligosaccharide glucanotransferase and cyclodextran by a protease-deficient Bacillus subtilis host-vector system (2012), Appl. Microbiol. Biotechnol., 93, 1877-1884.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.248 overexpression of CITase in Bacillus circulans strain G22-10, and, with the alpha-amylase promoter (PamyQ) and amyQ signal sequence of Bacillus amyloliquefaciens, in a protease-deficient Bacillus subtilis strain 168 as extracellular protein. The Bacillus subtilis host-vector system allows production of cycloisomaltooligosaccharides by direct fermentation of dextran along with high CITase production, which is not possible in Bacillus circulans G22-10 due to growth inhibition by dextran at high concentrations and limited production of CITase, stable expression up to 48 h Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.248 103000
-
recombinant CITAse, gel filtration Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.248 Niallia circulans P94286
-
-
2.4.1.248 Niallia circulans T-3040 P94286
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.248 recombinant extracellular CITase from protease-deficient Bacillus subtilis strain 168 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration and another step of anion exchange chromatography, recombinant extracellular CITase from Bacillus circulans G22-10 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.248 14.2
-
recombinant CITase from Bacillus circulans strain G22-10, pH 5.5, 40°C Niallia circulans
2.4.1.248 14.6
-
recombinant CITase from Bacillus subtilis strain 168 DELTAaprE DELTAnprE, pH 5.5, 40°C Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.248 CITase
-
Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.248 40
-
assay at Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.248 5.5
-
assay at Niallia circulans