EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.80 | drug development | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds, which can be then tested for their biological activities in drug discovery and development programs | Aspergillus fumigatus |
2.5.1.80 | synthesis | 7-DMATS catalyzes regio- and stereospecific prenylations and can be used as efficient catalysts for chemoenzymatic synthesis of prenylated compounds | Aspergillus fumigatus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.34 | recombinant expression | Aspergillus fumigatus |
2.5.1.80 | expression of His-tagged 7-DMATS in Escherichia coli strains XL1 Blue or M15 or in Saccharomyces cerevisiae strain INVSc1 | Aspergillus fumigatus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.34 | Mg2+ | required | Aspergillus fumigatus | |
2.5.1.80 | Mg2+ | required | Aspergillus fumigatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | - |
diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan | Aspergillus fumigatus | - |
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | Leptosphaeria maculans | - |
diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.34 | Aspergillus fumigatus | - |
- |
- |
2.5.1.80 | Aspergillus fumigatus | - |
- |
- |
2.5.1.122 | Leptosphaeria maculans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.34 | recombinant enzyme | Aspergillus fumigatus |
2.5.1.80 | recombinant His-tagged 7-DMATS from Escherichia coli strains XL1 Blue or M15 or from Saccharomyces cerevisiae strain INVSc1 by nickel affinity chromatography | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.34 | dimethylallyl diphosphate + 1-naphthol | - |
Aspergillus fumigatus | diphosphate + ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | L-tryptophan is the preferred substrate | Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + 1,6-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,6-diol | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + 1,7-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalene-1,7-diol | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + 1-naphthol | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-en-1-yl)naphthalen-1-ol | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + 2,6-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,6-diol | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | - |
Aspergillus fumigatus | diphosphate + 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 3-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol + 7-[(3-methylbut-2-en-1-yl)oxy]naphthalen-2-ol + 1,6-bis(3-methylbut-2-en-1-yl)naphthalene-2,7-diol | 1-(3-methylbut-2-en-1-yl)naphthalene-2,7-diol is the major product | ? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan | - |
? | |
2.5.1.80 | additional information | substrate specificity of 7-DMATS, overview | Aspergillus fumigatus | ? | - |
? | |
2.5.1.122 | dimethylallyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
2.5.1.122 | additional information | no activity with 1-naphthol, 1,6-dihydroxynaphthalene, 1,7-dihydroxynaphthalene, 2,6-dihydroxynaphthalene, 2,7-dihydroxynaphthalene, 3,5-dihydroxy-2-naphthoic acid, and 3,7-dihydroxy-2-naphthoic acid | Leptosphaeria maculans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.34 | FgaPT2 | - |
Aspergillus fumigatus |
2.5.1.80 | 7-dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
2.5.1.80 | 7-DMATS | - |
Aspergillus fumigatus |
2.5.1.122 | SirD | - |
Leptosphaeria maculans |
2.5.1.122 | tyrosine O-prenyltransferase | - |
Leptosphaeria maculans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.34 | 30 | - |
assay at | Aspergillus fumigatus |
2.5.1.80 | 30 | - |
assay at | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.34 | 7.5 | - |
assay at | Aspergillus fumigatus |
2.5.1.80 | 7.5 | - |
assay at | Aspergillus fumigatus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.34 | evolution | FgaPT2 belongs to the DMATS superfamily | Aspergillus fumigatus |
2.5.1.80 | evolution | the enzyme belongs to the DMATS superfamily | Aspergillus fumigatus |