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Literature summary extracted from

  • Rauch, B.; Pahlke, J.; Schweiger, P.; Deppenmeier, U.
    Characterization of enzymes involved in the central metabolism of Gluconobacter oxydans (2010), Appl. Microbiol. Biotechnol., 88, 711-718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.215 expression in Escherichia coli Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.215 0.01
-
 NADPH below 0.01 mM, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.215 0.051
-
 NADH pH 7.0, 25°C Gluconobacter oxydans
1.1.1.215 5.16
-
 2-oxo-D-gluconate pH 7.0, 25°C Gluconobacter oxydans
1.1.1.343 0.064
-
 NAD+ in 40 mM potassium phosphate buffer, at pH 7.0 and 25°C Gluconobacter oxydans
1.1.1.343 0.44
-
 6-phospho-D-gluconate in 40 mM potassium phosphate buffer, at pH 7.0 and 25°C Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 33200
-
 6 * 33200, SDS-PAGE Gluconobacter oxydans
1.1.1.215 200000
-
 PAGE Gluconobacter oxydans
1.1.1.343 35900
-
 4 * 35900, SDS-PAGE Gluconobacter oxydans
1.1.1.343 135000
-
 native PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 2-oxo-D-gluconate + NADPH + H+ Gluconobacter oxydans
-
 D-gluconate + NADP+
-
 ?
1.1.1.215 2-oxo-D-gluconate + NADPH + H+ Gluconobacter oxydans 621H
-
 D-gluconate + NADP+
-
 ?
1.1.1.215 2-oxo-L-gulonate + NADPH + H+ Gluconobacter oxydans
-
 L-idonate + NADP+
-
 ?
1.1.1.215 2-oxo-L-gulonate + NADPH + H+ Gluconobacter oxydans 621H
-
 L-idonate + NADP+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NAD+ Gluconobacter oxydans
-
 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Gluconobacter oxydans Q5FTU6
-
-
1.1.1.215 Gluconobacter oxydans 621H Q5FTU6
-
-
1.1.1.343 Gluconobacter oxydans G5EBD7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 2-oxo-D-gluconate + NADH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans D-gluconate + NAD+
-
 ?
1.1.1.215 2-oxo-D-gluconate + NADH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans 621H D-gluconate + NAD+
-
 ?
1.1.1.215 2-oxo-D-gluconate + NADPH + H+
-
 Gluconobacter oxydans D-gluconate + NADP+
-
 ?
1.1.1.215 2-oxo-D-gluconate + NADPH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans D-gluconate + NADP+
-
 ?
1.1.1.215 2-oxo-D-gluconate + NADPH + H+
-
 Gluconobacter oxydans 621H D-gluconate + NADP+
-
 ?
1.1.1.215 2-oxo-D-gluconate + NADPH + H+ enzyme shows dual cofactor specificity, being able to use both NADPH and NADH Gluconobacter oxydans 621H D-gluconate + NADP+
-
 ?
1.1.1.215 2-oxo-L-gulonate + NADPH + H+
-
 Gluconobacter oxydans L-idonate + NADP+
-
 ?
1.1.1.215 2-oxo-L-gulonate + NADPH + H+
-
 Gluconobacter oxydans 621H L-idonate + NADP+
-
 ?
1.1.1.343 6-phospho-D-gluconate + NAD+
-
 Gluconobacter oxydans D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 hexamer 6 * 33200, SDS-PAGE Gluconobacter oxydans
1.1.1.343 homotetramer 4 * 35900, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.215 Gox0417
-
 Gluconobacter oxydans
1.1.1.343 6PGDH
-
 Gluconobacter oxydans
1.1.1.343 Gox1705
-
 Gluconobacter oxydans
1.1.1.343 phosphogluconate dehydrogenase
-
 Gluconobacter oxydans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.215 48.2
-
 2-oxo-D-gluconate pH 7.0, 25°C Gluconobacter oxydans
1.1.1.343 23
-
 6-phospho-D-gluconate in 40 mM potassium phosphate buffer, at pH 7.0 and 25°C Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 NADH
-
 Gluconobacter oxydans
1.1.1.215 NADPH
-
 Gluconobacter oxydans
1.1.1.343 NAD+ dependent on Gluconobacter oxydans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.215 9.34
-
 2-oxo-D-gluconate pH 7.0, 25°C Gluconobacter oxydans
1.1.1.343 54.5
-
 6-phospho-D-gluconate in 40 mM potassium phosphate buffer, at pH 7.0 and 25°C Gluconobacter oxydans