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Literature summary extracted from
- Impact of malic enzymes on antibiotic and triacylglycerol production in Streptomyces coelicolor (2012), Appl. Environ. Microbiol., 78, 4571-4579.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.39 | gene Sco2951, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
| 1.1.1.40 | gene Sco5261, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.39 | 4.3 | - |
NAD+ | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor |  |
| 1.1.1.39 | 8.1 | - |
(S)-malate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
| 1.1.1.40 | 0.107 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
| 1.1.1.40 | 6.4 | - |
(S)-malate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
| 1.1.1.40 | 18.1 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.39 | Mg2+ | activates | Streptomyces coelicolor | |
| 1.1.1.40 | Mg2+ | activates | Streptomyces coelicolor | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.39 | (S)-malate + NAD+ | Streptomyces coelicolor | - |
pyruvate + CO2 + NADH | - |
? | |
| 1.1.1.39 | (S)-malate + NAD+ | Streptomyces coelicolor M145 | - |
pyruvate + CO2 + NADH | - |
? | |
| 1.1.1.40 | (S)-malate + NADP+ | Streptomyces coelicolor | - |
pyruvate + CO2 + NADPH | - |
r | |
| 1.1.1.40 | (S)-malate + NADP+ | Streptomyces coelicolor M145 | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.39 | Streptomyces coelicolor | - |
gene Sco2951 | - |
| 1.1.1.39 | Streptomyces coelicolor M145 | - |
gene Sco2951 | - |
| 1.1.1.40 | Streptomyces coelicolor | Q9F3K4 | gene Sco5261 | - |
| 1.1.1.40 | Streptomyces coelicolor M145 | Q9F3K4 | gene Sco5261 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.39 | (S)-malate + NAD+ | - |
Streptomyces coelicolor | pyruvate + CO2 + NADH | - |
? | |
| 1.1.1.39 | (S)-malate + NAD+ | - |
Streptomyces coelicolor M145 | pyruvate + CO2 + NADH | - |
? | |
| 1.1.1.40 | (S)-malate + NADP+ | - |
Streptomyces coelicolor | pyruvate + CO2 + NADPH | - |
r | |
| 1.1.1.40 | (S)-malate + NADP+ | - |
Streptomyces coelicolor M145 | pyruvate + CO2 + NADPH | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.39 | malic enzyme-NAD | - |
Streptomyces coelicolor |
| 1.1.1.39 | ME-NAD | - |
Streptomyces coelicolor |
| 1.1.1.40 | malic enzyme-NADP | - |
Streptomyces coelicolor |
| 1.1.1.40 | ME-NADP | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.39 | 30 | - |
assay at | Streptomyces coelicolor |
| 1.1.1.40 | 30 | - |
assay at | Streptomyces coelicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.39 | 8 | - |
assay at | Streptomyces coelicolor |
| 1.1.1.40 | 8 | - |
assay at | Streptomyces coelicolor |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.39 | NAD+ | dependent on | Streptomyces coelicolor | |
| 1.1.1.40 | NADP+ | dependent on | Streptomyces coelicolor | |
| 1.1.1.40 | NADPH | - |
Streptomyces coelicolor | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.39 | malfunction | the single Sco2951 and the double Sco2951 Sco5261 mutants, deficient in ME-NAD and ME-NADP, EC 1.1.1.40, activity, display a strong reduction in the production of the polyketide antibiotic actinorhodin. Additionally, the Sco2951/Sco5261 mutant shows a decrease in stored triacylglycerides during exponential growth | Streptomyces coelicolor |
| 1.1.1.39 | physiological function | the enzyme plays a role in antibiotic and triacylglycerol production, e.g. production of the polyketide antibiotic actinorhodin, overview | Streptomyces coelicolor |
| 1.1.1.40 | malfunction | the double Sco2951 Sco5261 mutant, deficient in ME-NAD, EC 1.1.1.39, and ME-NADP activity, display a strong reduction in the production of the polyketide antibiotic actinorhodin. Additionally, the Sco2951/Sco5261 mutant shows a decrease in stored triacylglcerides during exponential growth | Streptomyces coelicolor |
| 1.1.1.40 | physiological function | the enzyme plays a role in antibiotic and triacylglycerol production, e.g. production of the polyketide antibiotic actinorhodin, overview | Streptomyces coelicolor |
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