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Literature summary extracted from

  • Dai, Z.; Fu, H.; Zhang, Y.; Zeng, J.; Tang, B.; Tang, X.F.
    Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension (2012), Appl. Environ. Microbiol., 78, 4233-4241.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B55 the recombinant proform of Pyrococcus furiosus pyrolysin and several N- and C-terminal deletion mutants are successfully expressed in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B55 3.32
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme Pyrococcus furiosus
3.4.21.B55 3.83
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B55 Pyrococcus furiosus P72186
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B55 proteolytic modification the maturation of the enzyme involves autoprocessing of both the N-propeptide and a C-terminal propeptide. The long C-terminal extension can be dissected into a C-terminal propeptide and a C-terminal extension (CTEm) that is attached to the catalytic domain of the mature enzyme. Both of these propeptides assist in achieving pyrolysin hyperthermostability Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B55
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B55 azocasein + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.4.21.B55 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide + H2O
-
 Pyrococcus furiosus succinyl-Ala-Ala-Pro-Lys + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B55 90
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B55 1893
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) Pyrococcus furiosus
3.4.21.B55 3128
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B55 7.5
-
 assay at Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.B55 494
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) Pyrococcus furiosus
3.4.21.B55 942
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme Pyrococcus furiosus