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Literature summary extracted from

  • Tian, Y.S.; Xu, J.; Xiong, A.S.; Zhao, W.; Fu, X.Y.; Peng, R.H.; Yao, Q.H.
    Improvement of glyphosate resistance through concurrent mutations in three amino acids of the Ochrobactrum 5-enopyruvylshikimate-3-phosphate synthase (2011), Appl. Environ. Microbiol., 77, 8409-8414.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.19 agriculture Arabidopsis thaliana transgenic plants expressing mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L grow well in presence of 0.75 mM glyphosate, whereas Arabidopsis thaliana plants expressing wild-type enzyme grow only in presence of 0.25 mM glyphosate Brucella anthropi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression in Escherichia coli Brucella anthropi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.19 three-dimensional structure modeling of mutations P318R and M425T. Results indicate that the enzyme's lower hemisphere’s upper surface is possibly another important region for glyphosate resistance Brucella anthropi

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 E145G/N163H/N267S/P318R/M377V/M425T/P438L mutant is able to restore the growth of the Escherichia coli mutant ER2799, with the 5-enolpyruvylshikimate-3-phosphate synthase gene deleted from its genome, on an M9 minimal medium containing 300 mM glyphosate. The effectiveness of amino acid alterations N267S, P318R, and M245T on glyphosate tolerance is in the decreasing order P318R, M425T, N267S Brucella anthropi
2.5.1.19 M425T mutation identified in a multiple mutant enzyme resistant to glyphosate Brucella anthropi
2.5.1.19 N267S mutation identified in a multiple mutant enzyme resistant to glyphosate Brucella anthropi
2.5.1.19 N267S/P318R/M425T mutant enzyme shows high tolerance against glyphosate Brucella anthropi
2.5.1.19 P318R mutation identified in a multiple mutant enzyme resistant to glyphosate Brucella anthropi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 glyphosate
-
 Brucella anthropi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.23
-
 phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.25
-
 phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.31
-
 phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.32
-
 phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.38
-
 phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.525
-
 phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Brucella anthropi A6WUV8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Brucella anthropi phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.19 26.7
-
 phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 31.7
-
 phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 35
-
 phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 43.3
-
 phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 51.7
-
 phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 51.7
-
 phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.069
-
 glyphosate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.08
-
 glyphosate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.091
-
 glyphosate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.11
-
 glyphosate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.15
-
 glyphosate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 0.16
-
 glyphosate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.19 0.61
-
 wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
2.5.1.19 0.69
-
 mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
2.5.1.19 0.73
-
 mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
2.5.1.19 0.81
-
 mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
2.5.1.19 0.91
-
 mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
2.5.1.19 0.92
-
 mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.19 83.3
-
 phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 109.3
-
 phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 115.9
-
 phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 139.8
-
 phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 206.7
-
 phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi
2.5.1.19 206.7
-
 phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi