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Literature summary extracted from

  • Grunden, A.M.; Jenney, F.E.; Ma, K.; Ji, M.; Weinberg, M.V.; Adams, M.W.
    In vitro reconstitution of an NADPH-dependent superoxide reduction pathway from Pyrococcus furiosus (2005), Appl. Environ. Microbiol., 71, 1522-1530.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.4 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.4 0.0016
-
 oxidized rubredoxin pH 8, 23°C, cosubstrate: NADPH Pyrococcus furiosus
1.18.1.4 0.037
-
 oxidized rubredoxin pH 8, 80°C, cosubstrate: NADPH Pyrococcus furiosus
1.18.1.4 0.048
-
 NADPH pH 8, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
1.18.1.4 0.18
-
 NADH pH 8, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
1.18.1.4 5.5
-
 Oxidized benzyl viologen pH 8, 80°C, cosubstrate: NADH Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.4 39900
-
 x * 39900, calculated from sequence Pyrococcus furiosus
1.18.1.4 40000
-
 x * 40000, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + 2 H+ Pyrococcus furiosus superoxide reductase mediates reduction of superoxide to hydrogen peroxide in an NADPH-dependent manner via a coupled reaction between NAD(P)H:rubredoxin oxidoreductase, rubredoxin, and superoxide reductase rubredoxin + H2O2
-
 ?
1.18.1.4 2 oxidized rubredoxin + NADPH Pyrococcus furiosus superoxide reductase mediates reduction of superoxide to hydrogen peroxide in an NADPH-dependent manner via a coupled reaction between NAD(P)H:rubredoxin oxidoreductase, rubredoxin, and superoxide reductase 2 reduced rubredoxin + NADP+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.2 Pyrococcus furiosus
-
-
-
1.18.1.4 Pyrococcus furiosus Q8U1K9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.4
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + 2 H+
-
 Pyrococcus furiosus rubredoxin + H2O2
-
 ?
1.15.1.2 reduced rubredoxin + superoxide + 2 H+ superoxide reductase mediates reduction of superoxide to hydrogen peroxide in an NADPH-dependent manner via a coupled reaction between NAD(P)H:rubredoxin oxidoreductase, rubredoxin, and superoxide reductase Pyrococcus furiosus rubredoxin + H2O2
-
 ?
1.18.1.4 2 oxidized rubredoxin + NADPH
-
 Pyrococcus furiosus 2 reduced rubredoxin + NADP+ + H+
-
 ?
1.18.1.4 2 oxidized rubredoxin + NADPH superoxide reductase mediates reduction of superoxide to hydrogen peroxide in an NADPH-dependent manner via a coupled reaction between NAD(P)H:rubredoxin oxidoreductase, rubredoxin, and superoxide reductase Pyrococcus furiosus 2 reduced rubredoxin + NADP+ + H+
-
 ?
1.18.1.4 oxidized benzyl viologen + NADH
-
 Pyrococcus furiosus reduced benzyl viologen + NAD+ + H+
-
 ?
1.18.1.4 oxidized benzyl viologen + NADPH
-
 Pyrococcus furiosus reduced benzyl viologen + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.4 ? x * 40000, SDS-PAGE Pyrococcus furiosus
1.18.1.4 ? x * 39900, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.2 SOR
-
 Pyrococcus furiosus
1.18.1.4 NROR
-
 Pyrococcus furiosus
1.18.1.4 PF1197
-
 Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.4 258
-
 NADPH pH 8, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
1.18.1.4 350
-
 oxidized rubredoxin pH 8, 23°C, cosubstrate: NADPH Pyrococcus furiosus
1.18.1.4 498
-
 NADH pH 8, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
1.18.1.4 1236
-
 Oxidized benzyl viologen pH 8, 80°C, cosubstrate: NADH Pyrococcus furiosus
1.18.1.4 8980
-
 oxidized rubredoxin pH 8, 80°C, cosubstrate: NADPH Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.4 8
-
 assay at Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.4 FAD essential for activity, can not be replaced by FMN Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.4 225
-
 Oxidized benzyl viologen pH 8, 80°C, cosubstrate: NADH Pyrococcus furiosus
1.18.1.4 2770
-
 NADH pH 8, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
1.18.1.4 5375
-
 NADPH pH 8, 80°C, cosubstrate: benzyl viologen Pyrococcus furiosus
1.18.1.4 218900
-
 oxidized rubredoxin pH 8, 23°C, cosubstrate: NADPH Pyrococcus furiosus
1.18.1.4 242700
-
 oxidized rubredoxin pH 8, 80°C, cosubstrate: NADPH Pyrococcus furiosus