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Literature summary extracted from

  • Zhou, R.; Linz, J.E.
    Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus (1999), Appl. Environ. Microbiol., 65, 5639-5641.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B25 expression in Escherichia coli Aspergillus parasiticus
1.1.1.349 expressed in Escherichia coli DH5alpha cells Aspergillus parasiticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.349 32000
-
 x * 32000, SDS-PAGE, two recombinant Nor-1 proteins with apparent 32000 (major) and 36000 Da (minor) masses are detected Aspergillus parasiticus
1.1.1.349 36000
-
 x * 36000, SDS-PAGE, two recombinant Nor-1 proteins with apparent 32000 (major) and 36000 Da (minor) masses are detected Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B25 norsolorinate + NADPH + H+ Aspergillus parasiticus
-
 (1'S)-averantin + NADP+
-
 ?
1.1.1.B25 norsolorinate + NADPH + H+ Aspergillus parasiticus ATCC 56774
-
 (1'S)-averantin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B25 Aspergillus parasiticus
-
-
-
1.1.1.349 Aspergillus parasiticus
-
-
-
1.1.1.349 Aspergillus parasiticus ATCC 56774
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.349 amylose resin column chromatography Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B25 norsolorinate + NADPH + H+
-
 Aspergillus parasiticus (1'S)-averantin + NADP+
-
 ?
1.1.1.B25 norsolorinate + NADPH + H+
-
 Aspergillus parasiticus ATCC 56774 (1'S)-averantin + NADP+
-
 ?
1.1.1.349 norsolorinic acid + NADPH + H+
-
 Aspergillus parasiticus (1'S)-averantin + NADP+
-
 ?
1.1.1.349 norsolorinic acid + NADPH + H+
-
 Aspergillus parasiticus ATCC 56774 (1'S)-averantin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.349 ? x * 32000, SDS-PAGE, two recombinant Nor-1 proteins with apparent 32000 (major) and 36000 Da (minor) masses are detected Aspergillus parasiticus
1.1.1.349 ? x * 36000, SDS-PAGE, two recombinant Nor-1 proteins with apparent 32000 (major) and 36000 Da (minor) masses are detected Aspergillus parasiticus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B25 nor-1
-
 Aspergillus parasiticus
1.1.1.B25 nor-1 protein
-
 Aspergillus parasiticus
1.1.1.349 nor-1
-
 Aspergillus parasiticus
1.1.1.349 nor-1 protein
-
 Aspergillus parasiticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B25 NADPH
-
 Aspergillus parasiticus
1.1.1.349 NADPH
-
 Aspergillus parasiticus