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Literature summary extracted from

  • Yu, J.; Chang, P.K.; Ehrlich, K.C.; Cary, J.W.; Montalbano, B.; Dyer, J.M.; Bhatnagar, D.; Cleveland, T.E.
    Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2 (1998), Appl. Environ. Microbiol., 64, 4834-4841.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.117 expressed in Saccharomyces cerevisiae strain InvSc1 Aspergillus parasiticus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.117 A143S the mutation affects enzymatic activity (approximately 50% of the enzyme activity which converts O-methylsterigmatocystin to aflatoxin B1 is lost) Aspergillus parasiticus
1.14.14.117 H400L the mutation results in a loss of the monooxygenase activity Aspergillus parasiticus
1.14.14.117 I528Y the mutation does not affect enzymatic activity Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 143
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus
-
 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 143
-
 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.117 Aspergillus parasiticus O13345
-
-
1.14.14.117 Aspergillus parasiticus SRRC 143 O13345
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.117 mycelium
-
 Aspergillus parasiticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus SRRC 143 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
1.14.14.117 8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus SRRC 143 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.117 ordA
-
 Aspergillus parasiticus

General Information

EC Number General Information Comment Organism
1.14.14.117 metabolism the enzyme is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2 Aspergillus parasiticus