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Literature summary extracted from

  • Kristiansson, H.; Timson, D.J.
    N-acetylgalactosamine kinase: a naturally promiscuous small molecule kinase (2012), Appl. Biochem. Biotechnol., 166, 57-63.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.157 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.157 F444A mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
2.7.1.157 F444C mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme but increases turnover rate with N-acetylgalactosamine Homo sapiens
2.7.1.157 F444H mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
2.7.1.157 F444K mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
2.7.1.157 F444R mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens
2.7.1.157 F444S mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme but increases turnover rate with N-acetylgalactosamine Homo sapiens
2.7.1.157 F444W mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.157 0.006
-
 ATP mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.009
-
 ATP mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.009
-
 ATP wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.011
-
 ATP mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.013
-
 ATP mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.013
-
 ATP mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.015
-
 ATP mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.015
-
 ATP mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.015
-
 ATP mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.017
-
 ATP mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.017
-
 ATP mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.018
-
 ATP mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.018
-
 ATP wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.019
-
 ATP mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.024
-
 ATP mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.024
-
 ATP mutant F444W, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.025
-
 ATP mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine Homo sapiens
2.7.1.157 0.027
-
 ATP mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine Homo sapiens
2.7.1.157 0.028
-
 ATP mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.032
-
 ATP wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.039
-
 ATP mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.051
-
 N-acetyl-alpha-D-glucosamine wild-type, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.12
-
 N-acetyl-alpha-D-glucosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.12
-
 N-acetyl-alpha-D-glucosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.13
-
 ATP mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine Homo sapiens
2.7.1.157 0.17
-
 N-acetyl-alpha-D-galactosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.22
-
 N-acetyl-alpha-D-glucosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.24
-
 N-acetyl-alpha-D-galactosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.25
-
 N-acetyl-alpha-D-mannosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.27
-
 N-acetyl-alpha-D-galactosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.28
-
 N-acetyl-alpha-D-glucosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.33
-
 N-acetyl-alpha-D-galactosamine wild-type, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.34
-
 N-acetyl-alpha-D-galactosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.37
-
 N-acetyl-alpha-D-glucosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.39
-
 N-acetyl-alpha-D-glucosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.47
-
 N-acetyl-alpha-D-mannosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.56
-
 N-acetyl-alpha-D-galactosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.65
-
 N-acetyl-alpha-D-mannosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.69
-
 N-acetyl-alpha-D-galactosamine mutant F444W, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.79
-
 N-acetyl-alpha-D-mannosamine wild-type, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.93
-
 N-acetyl-alpha-D-mannosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.95
-
 N-acetyl-alpha-D-galactosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.95
-
 N-acetyl-alpha-D-mannosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
2.7.1.157 4.1
-
 N-acetyl-alpha-D-mannosamine mutant F444S, 37°C, pH 7.5 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.157 Homo sapiens Q01415 isoform GALK2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.157 ATP + N-acetyl-alpha-D-galactosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.157 ATP + N-acetyl-alpha-D-glucosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.1.157 ATP + N-acetyl-alpha-D-mannosamine
-
 Homo sapiens ADP + N-acetyl-alpha-D-mannosamine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.157 GALK2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.157 0.053
-
 N-acetyl-alpha-D-mannosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.063
-
 N-acetyl-alpha-D-glucosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.066
-
 N-acetyl-alpha-D-mannosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.071
-
 N-acetyl-alpha-D-glucosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.072
-
 N-acetyl-alpha-D-glucosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.087
-
 N-acetyl-alpha-D-glucosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.11
-
 N-acetyl-alpha-D-glucosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.16
-
 N-acetyl-alpha-D-glucosamine wild-type, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.18
-
 N-acetyl-alpha-D-glucosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.19
-
 N-acetyl-alpha-D-mannosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.19
-
 N-acetyl-alpha-D-mannosamine wild-type, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.23
-
 N-acetyl-alpha-D-mannosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.25
-
 N-acetyl-alpha-D-mannosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.4
-
 N-acetyl-alpha-D-mannosamine mutant F444S, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.62
-
 N-acetyl-alpha-D-galactosamine mutant F444W, 37°C, pH 7.5 Homo sapiens
2.7.1.157 0.72
-
 N-acetyl-alpha-D-galactosamine mutant F444R, 37°C, pH 7.5 Homo sapiens
2.7.1.157 1
-
 N-acetyl-alpha-D-galactosamine mutant F444K, 37°C, pH 7.5 Homo sapiens
2.7.1.157 1.2
-
 N-acetyl-alpha-D-galactosamine wild-type, 37°C, pH 7.5 Homo sapiens
2.7.1.157 1.6
-
 N-acetyl-alpha-D-galactosamine mutant F444H, 37°C, pH 7.5 Homo sapiens
2.7.1.157 1.9
-
 N-acetyl-alpha-D-galactosamine mutant F444A, 37°C, pH 7.5 Homo sapiens
2.7.1.157 2.6
-
 N-acetyl-alpha-D-galactosamine mutant F444C, 37°C, pH 7.5 Homo sapiens
2.7.1.157 3.2
-
 N-acetyl-alpha-D-galactosamine mutant F444S, 37°C, pH 7.5 Homo sapiens