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Literature summary extracted from

  • Van Horn, W.D.; Sanders, C.R.
    Prokaryotic diacylglycerol kinase and undecaprenol kinase (2012), Annu. Rev. Biophys., 41, 81-101.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.66 drug development the enzyme is a possible anti-cariogenic drug target Streptococcus mutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.66 plasma membrane located in the plasma membrane with the active site facing the cytosol Bacillus subtilis 5886
-
2.7.1.66 plasma membrane located in the plasma membrane with the active site facing the cytosol Escherichia coli 5886
-
2.7.1.66 plasma membrane located in the plasma membrane with the active site facing the cytosol Streptococcus mutans 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.66 Mg2+ required Bacillus subtilis
2.7.1.66 Mg2+ required Escherichia coli
2.7.1.66 Mg2+ required Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.66 17000
-
 1 * 17000 Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.66 ATP + undecaprenol Bacillus subtilis
-
 ADP + undecaprenyl phosphate
-
 ?
2.7.1.66 ATP + undecaprenol Escherichia coli
-
 ADP + undecaprenyl phosphate
-
 ?
2.7.1.66 ATP + undecaprenol Streptococcus mutans
-
 ADP + undecaprenyl phosphate
-
 ?
2.7.1.66 additional information Escherichia coli the diacylglycerol kinase of Escherichia coli shows very low undecaprenol kinase, a true undecaprenol kinase is not found in Escherichia coli ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.66 Bacillus subtilis
-
-
-
2.7.1.66 Escherichia coli
-
-
-
2.7.1.66 Streptococcus mutans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.66 native enzyme Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.66 ATP + undecaprenol
-
 Bacillus subtilis ADP + undecaprenyl phosphate
-
 ?
2.7.1.66 ATP + undecaprenol
-
 Escherichia coli ADP + undecaprenyl phosphate
-
 ?
2.7.1.66 ATP + undecaprenol
-
 Streptococcus mutans ADP + undecaprenyl phosphate
-
 ?
2.7.1.66 additional information the diacylglycerol kinase of Escherichia coli shows very low undecaprenol kinase, a true undecaprenol kinase is not found in Escherichia coli Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.66 monomer 1 * 17000 Streptococcus mutans

Synonyms

EC Number Synonyms Comment Organism
2.7.1.66 UDPK
-
 Bacillus subtilis
2.7.1.66 UDPK
-
 Escherichia coli
2.7.1.66 UDPK
-
 Streptococcus mutans

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.66 ATP
-
 Bacillus subtilis
2.7.1.66 ATP
-
 Escherichia coli
2.7.1.66 ATP
-
 Streptococcus mutans

General Information

EC Number General Information Comment Organism
2.7.1.66 evolution prokaryotic diacylglycerol kinase and undecaprenol kinase are the lone members of the prokaryotic diacylglycerol kinase and undecaprenol kinase family of multispan membrane enzymes that are very small, lack relationships to any other family of proteins, including water soluble kinases, and that exhibit an unusual structure and active site architecture. The enzymes show complex structure, forming domain-swapped homotrimers in which each monomer subunit has three transmembrane segments. Members of this family are unrelated to the eukaryotic diacylglycerol kinases and their prokaryotic homologs, encoded by the dgkB gene Streptococcus mutans
2.7.1.66 metabolism UDPK activity does not participate in the de novo biosynthetic pathway to undecaprenyl phosphate which does not include free undecaprenol as an intermediate Streptococcus mutans
2.7.1.66 physiological function Gram-negative Escherichia coli does not contain high llevels of free undecaprenol, thus has no need for a undecaprenol kinase Escherichia coli
2.7.1.66 physiological function UDPK is essential for sporulation of Bacillus subtilis Bacillus subtilis
2.7.1.66 physiological function UDPK seems to play an analogous role in Gram-positive bacteria, where its importance is evident by the fact that UDPK is essential for biofilm formation by the oral pathogen Streptococcus mutans. Undecaprenol has an unusual function as covalent transmembrane carriers of hydrophilic molecules from the inner to the outer leaflet of the plasma membrane for use in biosynthesis of molecules located in the cell wall or periplasm. UDPK is an important enzyme in Streptococcus mutans required for growth at low pH, for biosynthesis of certain lantibiotics, and for biofilm formation.. It is also a virulence factor for the formation of smooth surface dental caries Streptococcus mutans