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Literature summary extracted from

  • Yi, L.; Velasquez, M.S.; Holler, T.P.; Woodard, R.W.
    A simple assay for 3-deoxy-D-manno-octulosonate cytidylyltransferase and its use as a pathway screen (2011), Anal. Biochem., 416, 152-158.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.38 analysis adaptation of a simple colorimetric assay for diphosphate to the enzyme 3-deoxy-D-manno-octulosonate cytidylyltransferase. This assay can be combined with the malachite green assay for phosphate to form an assay system capable of determining phosphate and diphosphate in the same solution. The assay system has the potential for simultaneous screening of the 3-deoxy-D-manno-octulosonate biosynthesis pathway Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.38 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 0.28
-
 3-deoxy-D-manno-octulosonate in the presence of 10 mM Mg2+, at pH 9.3 and 37°C Escherichia coli
2.7.7.38 0.33
-
 CTP in the presence of 10 mM Mg2+, at pH 9.3 and 37°C Escherichia coli
2.7.7.38 0.52
-
 3-deoxy-D-manno-octulosonate in the presence of 5 mM Mg2+, at pH 10.0 and 37°C Escherichia coli
2.7.7.38 0.59
-
 CTP in the presence of 5 mM Mg2+, at pH 10.0 and 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Mg2+ optimum activity at 10 mM Mg2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.38 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
 Escherichia coli CMP-3-deoxy-D-manno-octulosonate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.38 3-deoxy-D-manno-octulosonate cytidylyltransferase
-
 Escherichia coli
2.7.7.38 CMP-KDO synthetase
-
 Escherichia coli
2.7.7.38 KdsB
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.38 12.5
-
 3-deoxy-D-manno-octulosonate in the presence of 10 mM Mg2+, at pH 9.3 and 37°C Escherichia coli
2.7.7.38 21.9
-
 3-deoxy-D-manno-octulosonate in the presence of 5 mM Mg2+, at pH 10.0 and 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.38 9.3
-
-
 Escherichia coli