BRENDA - Enzyme Database show

A fluorometric assay for high-throughput screening targeting nicotinamide phosphoribosyltransferase

Zhang, R.Y.; Qin, Y.; Lv, X.Q.; Wang, P.; Xu, T.Y.; Zhang, L.; Miao, C.Y.; Anal. Biochem. 412, 18-25 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.2.12
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
(4',5,7-trihydroxyflavone)-(3'->8)-(4',5,7-trihydroxyflavone)
-
Mus musculus
2.4.2.12
(4',7-dimethoxy-5-hydroxyflavone)-(3'->8)-(4',7-dimethoxy-5-hydroxyflavone)
-
Mus musculus
2.4.2.12
FK-866
i.e. (2E)-N-[4-(1-benzoylpiperidin-4-yl)butyl]-3-phenylprop-2-enamide
Mus musculus
2.4.2.12
gallotanine
i.e. (2R,3S,5R,6S)-tetrahydro-2H-pyran-2,3,4,5,6-pentaylpentakis(oxycarbonyl-5,6-dihydroxybenzene-3,1-diyl) pentakis(3,4,5-trihydroxybenzoate)
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.12
immobilized metal ion affinity chromatography
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phosphoribosyl 1-diphosphate
-
721273
Mus musculus
nicotinamide mononucleotide + diphosphate
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.4.2.12
0.0000009
-
i.e. (2E)-N-[4-(1-benzoylpiperidin-4-yl)butyl]-3-phenylprop-2-enamide, pH 7.5, 37°C
Mus musculus
FK-866
2.4.2.12
0.0013
-
i.e. (2R,3S,5R,6S)-tetrahydro-2H-pyran-2,3,4,5,6-pentaylpentakis(oxycarbonyl-5,6-dihydroxybenzene-3,1-diyl) pentakis(3,4,5-trihydroxybenzoate), pH 7.5, 37°C
Mus musculus
gallotanine
2.4.2.12
0.0066
-
pH 7.5, 37°C
Mus musculus
(4',5,7-trihydroxyflavone)-(3'->8)-(4',5,7-trihydroxyflavone)
2.4.2.12
0.0175
-
pH 7.5, 37°C
Mus musculus
(4',7-dimethoxy-5-hydroxyflavone)-(3'->8)-(4',7-dimethoxy-5-hydroxyflavone)
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL
Mus musculus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.4.2.12
0.0000009
-
i.e. (2E)-N-[4-(1-benzoylpiperidin-4-yl)butyl]-3-phenylprop-2-enamide, pH 7.5, 37°C
Mus musculus
FK-866
2.4.2.12
0.0013
-
i.e. (2R,3S,5R,6S)-tetrahydro-2H-pyran-2,3,4,5,6-pentaylpentakis(oxycarbonyl-5,6-dihydroxybenzene-3,1-diyl) pentakis(3,4,5-trihydroxybenzoate), pH 7.5, 37°C
Mus musculus
gallotanine
2.4.2.12
0.0066
-
pH 7.5, 37°C
Mus musculus
(4',5,7-trihydroxyflavone)-(3'->8)-(4',5,7-trihydroxyflavone)
2.4.2.12
0.0175
-
pH 7.5, 37°C
Mus musculus
(4',7-dimethoxy-5-hydroxyflavone)-(3'->8)-(4',7-dimethoxy-5-hydroxyflavone)
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
(4',5,7-trihydroxyflavone)-(3'->8)-(4',5,7-trihydroxyflavone)
-
Mus musculus
2.4.2.12
(4',7-dimethoxy-5-hydroxyflavone)-(3'->8)-(4',7-dimethoxy-5-hydroxyflavone)
-
Mus musculus
2.4.2.12
FK-866
i.e. (2E)-N-[4-(1-benzoylpiperidin-4-yl)butyl]-3-phenylprop-2-enamide
Mus musculus
2.4.2.12
gallotanine
i.e. (2R,3S,5R,6S)-tetrahydro-2H-pyran-2,3,4,5,6-pentaylpentakis(oxycarbonyl-5,6-dihydroxybenzene-3,1-diyl) pentakis(3,4,5-trihydroxybenzoate)
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
immobilized metal ion affinity chromatography
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phosphoribosyl 1-diphosphate
-
721273
Mus musculus
nicotinamide mononucleotide + diphosphate
-
-
-
?