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Literature summary extracted from

  • Orzechowski, S.; Grabowska, A.; Sitnicka, D.; Siminska, J.; Felus, M.; Dudkiewicz, M.; Fudali, S.; Sobczak, M.
    Analysis of the expression, subcellular and tissue localisation of phosphoglucan, water dikinase (PWD/GWD3) in Solanum tuberosum L.: A bioinformatics approach for the comparative analysis of two alpha-glucan, water dikinases (GWDs) from Solanum tuberosum L. (2013), Acta Physiol. Plant., 35, 483-500.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.5 expressed in Escherichia coli strain JM109 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.4 160000
-
-
 Solanum tuberosum
2.7.9.5 125000
-
 x * 125000, SDS-PAGE Solanum tuberosum
2.7.9.5 132270
-
 x * 132270, calculated from amino acid sequence Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.4 Solanum tuberosum
-
-
-
2.7.9.5 Solanum tuberosum D2JRZ6 cultivar Russet Burbank
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.5 ammonium sulfate precipitation, amylose resin column chromatography, and Sephadex G-25 gel filtration Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.4 leaf
-
 Solanum tuberosum
-
2.7.9.4 tuber
-
 Solanum tuberosum
-
2.7.9.5 leaf
-
 Solanum tuberosum
-
2.7.9.5 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.4 ATP + starch + H2O
-
 Solanum tuberosum AMP + phosphorylated starch + phosphate
-
 ?
2.7.9.5 ATP + [phospho-alpha-glucan] + H2O
-
 Solanum tuberosum AMP + O-phospho-[phospho-alpha-glucan] + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.5 ? x * 125000, SDS-PAGE Solanum tuberosum
2.7.9.5 ? x * 132270, calculated from amino acid sequence Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
2.7.9.4 GWD1
-
 Solanum tuberosum
2.7.9.5 GWD3 isoform Solanum tuberosum
2.7.9.5 PWD isoform Solanum tuberosum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.9.5 Solanum tuberosum calculated from amino acid sequence
-
 5.8

Expression

EC Number Organism Comment Expression
2.7.9.4 Solanum tuberosum isoform GWD1 expression reaches its maximum after 10 h of illumination, The lowest expression of isoform GWD1 occurs 4 h after turning the light on, and it is approximately 7times lower than the expression measured 6 h late up
2.7.9.5 Solanum tuberosum the lowest level isoform GWD3 expression is 4 h after turning the light off, and it was more than 2.5times lower than the maximal expression level down
2.7.9.5 Solanum tuberosum the expression of isoform GWD3 during the diurnal cycle increases until 13 h of illumination, when it reaches its maximal level up

General Information

EC Number General Information Comment Organism
2.7.9.4 metabolism GWD1 phosphorylates highly ordered, insoluble starch, and glucan phosphorylation at the C-6 position results in a transition state of the phosphoglucans,´which is less ordered but still insoluble. This specific state of the starch granule is an appropriate substrate for phosphoglucan, water dikinase GWD3 catalysis (C-3 phosphorylation) after which the phosphoglucan finally becomes soluble Solanum tuberosum