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Literature summary extracted from

  • Satomura, T.; Hiraki, A.; Kawai, T.; Kawakami, R.; Ohshima, T.; Sakuraba, H.
    Expression, purification, crystallization and preliminary X-ray diffraction analysis of a galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2012), Acta Crystallogr. Sect. F, 68, 330-332.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.12 gene PAE1184, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Codon Plus RIL Pyrobaculum aerophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.12 purified recombinant enzyme, sitting-drop vapour-diffusion method, 10mg/ml protein, 4°C, X-ray diffraction structure determination and analysis at 2.73 A resolution Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 36000
-
 2 * 36000, SDS-PAGE Pyrobaculum aerophilum
2.7.7.12 59000
-
 gel filtration Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Pyrobaculum aerophilum
-
 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate Pyrobaculum aerophilum DSM 7523
-
 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Pyrobaculum aerophilum Q8ZXN7
-
-
2.7.7.12 Pyrobaculum aerophilum DSM 7523 Q8ZXN7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 recombinant enzyme from Escherichia coli strain BL21 (DE3) Codon Plus RIL by heat treatment at 80°C for 10 min, followed by anion exchange and cation exchange chromatography Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Pyrobaculum aerophilum alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate
-
 Pyrobaculum aerophilum DSM 7523 alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 homodimer 2 * 36000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

EC Number Synonyms Comment Organism
2.7.7.12 galactose-1-phosphate uridylyltransferase
-
 Pyrobaculum aerophilum
2.7.7.12 GALT
-
 Pyrobaculum aerophilum

General Information

EC Number General Information Comment Organism
2.7.7.12 metabolism the enzyme catalyzes a pivotal step in the Leloir pathway of galactose metabolism, overview Pyrobaculum aerophilum