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Literature summary extracted from

  • Zhuang, N.; Seo, K.H.; Chen, C.; Zhou, J.; Kim, S.W.; Lee, K.H.
    Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei (2012), Acta Crystallogr. Sect. F, 68, 1560-1563.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expressed in Escherichia coli BL21(DE3) cells Methanosarcina mazei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.36 hanging drop vapor diffusion method, using 0.32 M MgCl2, 0.08 M bis-Tris pH 5.5, 16% (w/v) PEG 3350, at 18°C Methanosarcina mazei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 33000
-
 2 * 33000, SDS-PAGE Methanosarcina mazei
2.7.1.36 33600
-
 2 * 33600, His-tagged protein, calculated from amino acid sequence Methanosarcina mazei
2.7.1.36 64600
-
 gel filtration Methanosarcina mazei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Methanosarcina mazei Q8PW39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 Ni-NTA bead column chromatography, Resource 15Q column chromatography, and Superdex 200 gel filtration Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Methanosarcina mazei ADP + (R)-5-phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 homodimer 2 * 33000, SDS-PAGE Methanosarcina mazei
2.7.1.36 homodimer 2 * 33600, His-tagged protein, calculated from amino acid sequence Methanosarcina mazei

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 MVK
-
 Methanosarcina mazei