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Literature summary extracted from

  • Quirk, S.
    Crystallization and preliminary X-ray characterization of the Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase (2012), Acta Crystallogr. Sect. F, 68, 1109-1112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.52 expression as fusion protein with maltose-binding protein in Escherichia coli strain BL21 (DE3) Vitis vinifera
2.7.7.30 expresssion in Escherichia coli Vitis vinifera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.52 purified apo-enzyme, mixing of 10 mg/ml protein in 10 mM Tris-HCl pH 7.5 with 9% w/v PEG 6K, 5% v/v ethylene glycol, 5% v/v glycerol, 25 mM HEPES pH 7.5, 2% tert-butanol, 1 mM MgCl2, 1mM MnCl2, 24°C, 20-30 days, X-ray diffraction structure determination and analysis at 2.6 A resolution Vitis vinifera
2.7.7.30 to 2.6 A resolution. Space group P41212, a single enzyme monomer per asymmetric unit Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 0.8
-
 ATP pH and temperature not specified in the publication Vitis vinifera
2.7.7.30 0.2
-
 GTP pH not specified in the publication, temperature not specified in the publication Vitis vinifera
2.7.7.30 0.8
-
 ATP pH not specified in the publication, temperature not specified in the publication Vitis vinifera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.52 Mg2+ required Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.52 118800
-
 gel filtration Vitis vinifera
2.7.7.30 118800
-
 x * 118800, calculated Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + L-fucose Vitis vinifera
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 additional information Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Vitis vinifera
-
-
-
2.7.7.30 Vitis vinifera D7UCW9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.52 recombinant maltose-binding protein fusion protein from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, cleavage of the tag by Genease I protease, followed by guanosine affinity chromatography and ultrafiltration Vitis vinifera
2.7.7.30 recombinant protein with maltose-binding protein affinity tag Vitis vinifera

Storage Stability

EC Number Storage Stability Organism
2.7.7.30 -20°C, recombinant protein with maltose-binding protein affinity tag, stable for at least three months Vitis vinifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + L-fucose
-
 Vitis vinifera ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 additional information fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar Vitis vinifera ?
-
 ?
2.7.7.30 ATP + beta-L-fucose 1-phosphate
-
 Vitis vinifera ADP-L-fucose + diphosphate
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Vitis vinifera GDP-L-fucose + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.52 monomer 1 * 118800 Vitis vinifera
2.7.7.30 ? x * 118800, calculated Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
2.7.1.52 FKP
-
 Vitis vinifera
2.7.1.52 fucokinase:GDP-fucose pyrophosphorylase
-
 Vitis vinifera
2.7.1.52 L-fucokinase:GDP-fucose pyrophosphorylase
-
 Vitis vinifera
2.7.7.30 FKP
-
 Vitis vinifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.52 0.22
-
 ATP pH and temperature not specified in the publication Vitis vinifera
2.7.7.30 0.22
-
 ATP pH not specified in the publication, temperature not specified in the publication Vitis vinifera
2.7.7.30 1.55
-
 GTP pH not specified in the publication, temperature not specified in the publication Vitis vinifera

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.52 ATP
-
 Vitis vinifera