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Literature summary extracted from

  • Wang, X.; Akasaka, R.; Takemoto, C.; Morita, S.; Yamaguchi, M.; Terada, T.; Shirozu, M.; Yokoyama, S.; Chen, S.; Si, S.; Xie, Y.
    Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3 (2011), Acta Crystallogr. Sect. F, 67, 1551-1555.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.4 expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.4 sitting-drop vapour-diffusion method. Crystal belong to the trigonal space group P3(2)12, with unit-cell parameters a = b = 57.2, c = 107.9 A. There is a single molecule in the asymmetric unit Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.4 80000
-
 1 * 80000, the enzyme mainly exists as dimer in solution, SDS-PAGE Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.4 Pyrococcus horikoshii O58187
-
-
6.3.4.4 Pyrococcus horikoshii OT-3 O58187
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.4
-
 Pyrococcus horikoshii

Subunits

EC Number Subunits Comment Organism
6.3.4.4 dimer 1 * 80000, the enzyme mainly exists as dimer in solution, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.3.4.4 AdSS
-
 Pyrococcus horikoshii