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Literature summary extracted from

  • Van Hoorebeke, A.; Stout, J.; Van der Meeren, R.; Kyndt, J.; Van Beeumen, J.; Savvides, S.N.
    Crystallization and X-ray diffraction studies of inverting trehalose phosphorylase from Thermoanaerobacter sp. (2010), Acta Crystallogr. Sect. F, 66, 442-447.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.64 expressed in Escherichia coli BL21(DE3) cells Thermoanaerobacter brockii subsp. brockii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.64 hanging drop vapor diffusion method, using either 10% (w/v) PEG 4000, 0.1 M Tris-HCl pH 7.5, and 1% (v/v) 2-propanol or 10% (w/v) PEG 4000, 0.1 M Tris-HCl pH 7.5, and 1% (w/v) n-octyl-beta-D-glucoside Thermoanaerobacter brockii subsp. brockii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.64 84995
-
 2 * 84995, calculated from amino acid sequence Thermoanaerobacter brockii subsp. brockii
2.4.1.64 90000
-
 2 * 90000, SDS-PAGE Thermoanaerobacter brockii subsp. brockii
2.4.1.64 180000
-
 gel filtration Thermoanaerobacter brockii subsp. brockii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.64 Thermoanaerobacter brockii subsp. brockii
-
-
-
2.4.1.64 Thermoanaerobacter brockii subsp. brockii ATCC 33075
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.64 Ni-Sepharose column chromatography and Superdex 200 gel filtration Thermoanaerobacter brockii subsp. brockii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.64 alpha,alpha-trehalose + phosphate
-
 Thermoanaerobacter brockii subsp. brockii beta-D-glucose 1-phosphate + D-glucose
-
 r
2.4.1.64 alpha,alpha-trehalose + phosphate
-
 Thermoanaerobacter brockii subsp. brockii ATCC 33075 beta-D-glucose 1-phosphate + D-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.64 homodimer 2 * 90000, SDS-PAGE Thermoanaerobacter brockii subsp. brockii
2.4.1.64 homodimer 2 * 84995, calculated from amino acid sequence Thermoanaerobacter brockii subsp. brockii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.64 trehalose phosphorylase
-
 Thermoanaerobacter brockii subsp. brockii