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Literature summary extracted from

  • Balogun, E.O.; Inaoka, D.K.; Kido, Y.; Shiba, T.; Nara, T.; Aoki, T.; Honma, T.; Tanaka, A.; Inoue, M.; Matsuoka, S.; Michels, P.A.; Harada, S.; Kita, K.
    Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase (2010), Acta Crystallogr. Sect. F, 66, 304-308.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.30 expressed in Escherichia coli JM109 (DE3 + pRARE2) cells Trypanosoma brucei gambiense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.30 sitting drop vapor diffusion method, using 30% (w/v) PEG 400 and 100 mM HEPES pH 7.0, at 20°C Trypanosoma brucei gambiense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.30 glycosome
-
 Trypanosoma brucei gambiense 20015
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 60400
-
 x * 60400, calculated from amino acid sequence Trypanosoma brucei gambiense

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Trypanosoma brucei gambiense D3KVM3
-
-
2.7.1.30 Trypanosoma brucei gambiense IL2343 D3KVM3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30 Ni-NTA agarose column chromatography and Superdex 200 gel filtration Trypanosoma brucei gambiense

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma brucei gambiense ATP + glycerol
-
 r
2.7.1.30 ADP + sn-glycerol 3-phosphate
-
 Trypanosoma brucei gambiense IL2343 ATP + glycerol
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.30 ? x * 60400, calculated from amino acid sequence Trypanosoma brucei gambiense

General Information

EC Number General Information Comment Organism
2.7.1.30 physiological function the enzyme is essential for energy metabolism Trypanosoma brucei gambiense