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Literature summary extracted from

  • Kanaujia, S.P.; Ranjani, C.V.; Jeyakanthan, J.; Ohmori, M.; Agari, K.; Kitamura, Y.; Baba, S.; Ebihara, A.; Shinkai, A.; Kuramitsu, S.; Shiro, Y.; Sekar, K.; Yokoyama, S.
    Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of molybdopterin synthase from Thermus thermophilus HB8 (2007), Acta Crystallogr. Sect. F, 63, 324-326.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.12 expression of MoaB in Escherichia coli strain BL21(DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.12 purified MoaB, sitting drop vapour diffusion method, 0.001 ml of 11 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, are mixed with 0.001 ml of well solution containing 20% w/v PEG 3350, and 0.2 M tripotassium citrate monohydrate, 20°C, 2 weeks, X-ray diffraction structure determination and analysis at 1.64 A resolution Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.12 Thermus thermophilus
-
-
-
2.8.1.12 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.12 recombinaant MoaB from Escherichia coli strain BL21(DE3) by ultracentrifugation, hydrophobic interaction chromatography, gel filtration, anion and cation exchange chromatography, an hydroxyapatite chromatography Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.8.1.12 MoaB
-
 Thermus thermophilus
2.8.1.12 TTHA0341
-
 Thermus thermophilus