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Literature summary extracted from

  • Ng, C.L.; Waterman, D.G.; Antson, A.A.; Ortiz-Lombardia, M.
    Structure of the Methanothermobacter thermautotrophicus exosome RNase PH ring (2010), Acta Crystallogr. Sect. D, 66, 522-528.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.56 genes mth682 and mth683 encode the mthRrp41-mthRrp42 protein complex, cloning and recombinant expression of the His-tagged protein complex in Escherichia coli strain E2566 Methanothermobacter thermautotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.56 purified recombinant mthRrp41-mthRrp42 protein complex, hanging-drop vapour diffusion, 0.001 ml of protein in 25 mM Tris pH 7.5, 0.15 M NaCl, mixed with 0.001 ml of reservoir solution containing 5% 2-propanol, 20% PEG 3350, and 0.1 M succinic acid pH 7.0, X-ray diffraction structure determination and analysis at 2.65 A resolution Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.56 cytosol
-
 Methanothermobacter thermautotrophicus 5829
-
2.7.7.56 exosome the core of the exosome is a versatile multisubunit RNA processing enzyme found in archaea and eukaryotes, which includes a ring of six RNase PH subunits Methanothermobacter thermautotrophicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.56 Methanothermobacter thermautotrophicus
-
 genes mth682 and mth683
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.56 recombinant His-tagged mthRrp41-mthRrp42 protein complex from Escherichi coli strain E2566 by nickel affinity chromatography and ultrafiltration Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.56 additional information the reaction proceeds via strongly bound phosphate ions in the phosphorolytic active sites of mthRrp41 protein Methanothermobacter thermautotrophicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.56 hexamer multisubunit RNA processing enzyme including a ring of six RNase PH subunits Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.56 RNase PH
-
 Methanothermobacter thermautotrophicus
2.7.7.56 RNase PH ring
-
 Methanothermobacter thermautotrophicus

General Information

EC Number General Information Comment Organism
2.7.7.56 additional information the core of the exosome is a versatile multisubunit RNA processing enzyme found in archaea and eukaryotes, which includes a ring of six RNase PH subunits, all six RNase PH monomers are catalytically active in the homohexameric RNase PH. Modeling of the Mth exosome RNase PH ring, overview Methanothermobacter thermautotrophicus