EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.158 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparison of caffeine synthetic enzymes from coffee | Coffea sp. |
2.1.1.159 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparisonof caffeine synthetic enzymes from coffee | Coffea sp. |
2.1.1.160 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparisonof caffeine synthetic enzymes from coffee | Coffea sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.158 | A23S | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | A23S/L191P/H219R | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | A23S/P104Q/H219R | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | A23S/P104Q/Q161H/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows 60% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | A23S/P104Q/Q161H/L191P/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | A23S/Q161H/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | A23S/Q161H/L191P/H219R | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | H219R | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | L191P | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | additional information | construction of several enzyme mutants, overview. The mutants of CmXRS1, that have 3-N methylation activity and produce caffeine from paraxanthine as a substrate, need to have replacement of the glutamine residue by histidine at position 161 in the CmXRS1 sequence, i.e. a Q161H mutation, overview | Coffea sp. |
2.1.1.158 | P104Q | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | P104Q/L191P | site-directed mutagenesis, the mutant exhibits no 3-N methylation activity like the wild-type, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | P104Q/Q161H | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | P104Q/Q161H/L191P | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | Q161H | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.158 | Q161H/L191P | site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity | Coffea sp. |
2.1.1.159 | additional information | construction of several enzyme mutants, overview | Coffea sp. |
2.1.1.160 | additional information | construction of several enzyme mutants, overview | Coffea sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.158 | Mg2+ | required | Coffea sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
? | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 1,7-dimethylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 7-methylxanthine | Coffea sp. | - |
S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.158 | Coffea sp. | - |
- |
- |
2.1.1.159 | Coffea sp. | - |
- |
- |
2.1.1.160 | Coffea sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.158 | additional information | 7-methylxanthosine synthase shows the most critical substrate specifi city of the three types of N-methyltransferases in Coffea species | Coffea sp. | ? | - |
? | |
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
? | |
2.1.1.159 | additional information | minor activity of 3-N-methylation of paraxanthine is present in theobromine synthase | Coffea sp. | ? | - |
? | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? | |
2.1.1.160 | additional information | the descending order of activity of the purine base with caffeine synthase is N-3, N-1, N-7 | Coffea sp. | ? | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 1,7-dimethylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 1,7-dimethylxanthine | paraxanthine is the best methyl acceptor for caffeine synthase | Coffea sp. | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | - |
? | |
2.1.1.160 | S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea sp. | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.158 | CmXRS1 | - |
Coffea sp. |
2.1.1.159 | 3-N-methyltransferase | - |
Coffea sp. |
2.1.1.159 | CTS1 | - |
Coffea sp. |
2.1.1.159 | CTS2 | - |
Coffea sp. |
2.1.1.160 | CCS1 | - |
Coffea sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.158 | 27 | - |
assay at | Coffea sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.158 | 7.5 | - |
assay at | Coffea sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.158 | S-adenosyl-L-methionine | - |
Coffea sp. | |
2.1.1.159 | S-adenosyl-L-methionine | - |
Coffea sp. | |
2.1.1.160 | S-adenosyl-L-methionine | - |
Coffea sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.158 | metabolism | the enzyme catalyzes the first step in caffeine biosynthesis, pathway overview | Coffea sp. |
2.1.1.159 | metabolism | the enzyme catalyzes the second step in caffeine biosynthesis, pathway overview | Coffea sp. |
2.1.1.160 | metabolism | the enzyme catalyzes the second and third step in caffeine biosynthesis, pathway overview | Coffea sp. |