EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.72 | additional information | the enzyme is a direct-acting thrombolytic agent and does not rely on plasminogen for clot dissolution | Agkistrodon contortrix contortrix |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.24.72 | medicine | the use of the recombinant fibrolase alfimeprase results in rapid restoration of arterial patency in less than 4 h in most peripheral arterial occlusion patients | Agkistrodon contortrix contortrix |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.72 | expressed in Pichia pastoris | Agkistrodon contortrix contortrix |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.72 | alpha2-Macroglobulin | - |
Agkistrodon contortrix contortrix | |
3.4.24.72 | EDTA | - |
Agkistrodon contortrix contortrix |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.72 | Zn2+ | a zinc metalloproteinase containing one mole of zinc | Agkistrodon contortrix contortrix |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.72 | 23000 | - |
x * 23000 | Agkistrodon contortrix contortrix |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.72 | bradykinin + H2O | Agkistrodon contortrix contortrix | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg | - |
? | |
3.4.24.72 | Fibrin + H2O | Agkistrodon contortrix contortrix | fibrinolase cleaves the alpha-chain more rapidly than the beta-chain of fibrin | ? | - |
? | |
3.4.24.72 | Fibrinogen + H2O | Agkistrodon contortrix contortrix | fibrolase rapidly cleaves the A(alpha)-chain of fibrinogen and the B(beta)-chain at a slower rate, while it has no activity on the gamma-chain. The primary cleavage site at the alpha-chain ist he Lys-Leu bond at residues 413-414 | ? | - |
? | |
3.4.24.72 | low molecular weight kininogen + H2O | Agkistrodon contortrix contortrix | - |
kallidin + ? | i.e. Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | ? | |
3.4.24.72 | additional information | Agkistrodon contortrix contortrix | fibrolase neither activates nor degrades plasminogen | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.72 | Agkistrodon contortrix contortrix | - |
southern copperhead snake | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.72 | hydrophobic interaction HPLC, hydroxyapatite HPLC and cation exchange HPLC | Agkistrodon contortrix contortrix |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.72 | venom | - |
Agkistrodon contortrix contortrix | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.72 | bradykinin + H2O | - |
Agkistrodon contortrix contortrix | Arg-Pro-Pro-Gly-Phe-Ser-Pro + Phe-Arg | - |
? | |
3.4.24.72 | Fibrin + H2O | fibrinolase cleaves the alpha-chain more rapidly than the beta-chain of fibrin | Agkistrodon contortrix contortrix | ? | - |
? | |
3.4.24.72 | Fibrinogen + H2O | fibrolase rapidly cleaves the A(alpha)-chain of fibrinogen and the B(beta)-chain at a slower rate, while it has no activity on the gamma-chain. The primary cleavage site at the alpha-chain ist he Lys-Leu bond at residues 413-414 | Agkistrodon contortrix contortrix | ? | - |
? | |
3.4.24.72 | low molecular weight kininogen + H2O | - |
Agkistrodon contortrix contortrix | kallidin + ? | i.e. Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | ? | |
3.4.24.72 | additional information | fibrolase neither activates nor degrades plasminogen | Agkistrodon contortrix contortrix | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.72 | ? | x * 23000 | Agkistrodon contortrix contortrix |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.72 | Alfimeprase | commercial preparation. Alfimeprase is identical to fibrolase except for a two amino acid truncation at the amino-terminus and the insertion of a new amino-terminal amino acid in the truncated protein, these changes lead to a more stable enzyme for prolonged storage | Agkistrodon contortrix contortrix |