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Literature summary extracted from

  • Ding, L.; Saunders, R.J.; Drobna, Z.; Walton, F.S.; Xun, P.; Thomas, D.J.; Styblo, M.
    Methylation of arsenic by recombinant human wild-type arsenic (+3 oxidation state) methyltransferase and its methionine 287 threonine (M287T) polymorph: Role of glutathione (2012), Toxicol. Appl. Pharmacol., 264, 121-130.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.137 glutathione 1 mM GSH decreases Km and increases Vmax estimates for arsenite and methylarsonous acid Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.137 M287T in the absence of glutathione, the mutant shows decreased Vmax and Km for arsenite and increased Vmax and Km for methylarsonate compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.137 glutathione addition of 20 mM GSH sharply inhibits methylation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.137 0.0007
-
 methylarsonate wild type enzyme, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
2.1.1.137 0.0009
-
 methylarsonate mutant enzyme M287T, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
2.1.1.137 0.0015
-
 methylarsonate wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
2.1.1.137 0.0016
-
 arsenite mutant enzyme M287T, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens
2.1.1.137 0.0016
-
 arsenite wild type enzyme, in the presence of 1 mM glutathione, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens
2.1.1.137 0.002
-
 arsenite mutant enzyme M287T, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens
2.1.1.137 0.003
-
 methylarsonate mutant enzyme M287T, in 100 mM Tris-HCl buffer (pH 7.4), at 25°C Homo sapiens
2.1.1.137 0.0041
-
 arsenite wild type enzyme, in 100 mM Tris-HCl buffer (pH 7.4), at 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.137 Ni-NTA agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
 Homo sapiens S-adenosyl-L-homocysteine + methylarsonate + dimethylarsinous acid + dimethylarsinic acid
-
 ?
2.1.1.137 S-adenosyl-L-methionine + methylarsonate
-
 Homo sapiens S-adenosyl-L-homocysteine + dimethylarsinous acid + dimethylarsinic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.137 arsenic (+3 oxidation state) methyltransferase
-
 Homo sapiens
2.1.1.137 AS3MT
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.1.137 metabolism key enzyme in the pathway for methylation of arsenicals Homo sapiens